Comparison of Arbuscular Mycorrhizal Fungal Community in Roots and Rhizosphere of Invasive Cenchrus incertus and Native Plant in Inner Mongolia,China  

作　　者：Dan XIANG Baodong CHEN Huan LI Ruojuan LI Xin ZHANG 

机构地区：[1]State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]College of Resources and Environment,Qingdao Agricultural University,Qingdao 266109,China [3]Yunnan Forestry Technological College,Kunming 650224,China

出　　处：《Agricultural Science & Technology》2016年第3期655-661,共7页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(41071178,41101245);Knowledge Innovation Program of the Chinese Academy of Sciences(KZCX2-YW-BR-17)

摘　　要：Plant invasions could significantly alter arbuscular mycorrhizal(AM) fungal communities, but the effect may vary with plant species and local environments. Identifying changes in the AM fungal community due to plant invasion could improve our understanding of the invasion processes. Here, we examined the AM fungal community composition both in roots and rhizosphere soils of the invasive plant Cenchrus incertus and the dominant native plant Setaria viridis in a typical steppe in Inner Mongolia by using terminal restriction fragment length polymorphism analyses(T-RFLP). The results showed that AM fungal abundance in the rhizosphere soils of C. incertus was significantly lower than that of S. viridis. The AM fungal community composition in the rhizosphere soils of the two plant species also largely differed. In general, AM fungal community structures in roots corresponded very well to that in rhizosphere soils for both plant species. The dominant AM fungal type both in invasive and native plants was T-RFLP 524 bp, which represents Glomus sp.(Virtual taxa 109 and 287). Three specific T-RF types(280,190 and 141bp) were significantly more abundant in C. incertus, representing three clusters in Glomus which also named as VT(virtual taxa) 287, 64 and 214, Rhizophagus intraradices(VT 113) and Diversispora sp.(VT 60). While the specific T-RF types,189 and 279 bp, for S. viridis, only existed in Glomus cluster 1(VT 156), were significantly less abundant in C. incertus. These results indicated that AM fungi might play an important role in the invasion process of C. incertus, which still remains to be further investigated.Plant invasions could significantly alter arbuscular mycorrhizal（AM） fungal communities, but the effect may vary with plant species and local environments. Identifying changes in the AM fungal community due to plant invasion could improve our understanding of the invasion processes. Here, we examined the AM fungal community composition both in roots and rhizosphere soils of the invasive plant Cenchrus incertus and the dominant native plant Setaria viridis in a typical steppe in Inner Mongolia by using terminal restriction fragment length polymorphism analyses（T-RFLP）. The results showed that AM fungal abundance in the rhizosphere soils of C. incertus was significantly lower than that of S. viridis. The AM fungal community composition in the rhizosphere soils of the two plant species also largely differed. In general, AM fungal community structures in roots corresponded very well to that in rhizosphere soils for both plant species. The dominant AM fungal type both in invasive and native plants was T-RFLP 524 bp, which represents Glomus sp.（Virtual taxa 109 and 287）. Three specific T-RF types（280,190 and 141bp） were significantly more abundant in C. incertus, representing three clusters in Glomus which also named as VT（virtual taxa） 287, 64 and 214, Rhizophagus intraradices（VT 113） and Diversispora sp.（VT 60）. While the specific T-RF types,189 and 279 bp, for S. viridis, only existed in Glomus cluster 1（VT 156）, were significantly less abundant in C. incertus. These results indicated that AM fungi might play an important role in the invasion process of C. incertus, which still remains to be further investigated.

关 键 词：Arbuscular mycorrhiza fungi Cenchrus incertus Plant invasion T-RFLP qPCR 

分 类 号：S154.3[农业科学—土壤学]