γ-生育三烯酚抑制SGC-7901细胞上皮间质转化作用机制探讨  

Inhibitory Effects of γ- tocotrienol on Epithelial- mesenchymal Transition in Human Gastric Cancer SGC- 7901 Cells and Its Mechanism

作　　者：马可[1] 梁巍[2] 赵立东[1] 姜佳佳[2] 孙文广[3] 郭晓微[2] 

机构地区：[1]上海交通大学附属第六人民医院东院急诊医学科,201306 [2]哈尔滨医科大学附属第一临床医学院临床营养科,150001 [3]上海交通大学附属第六人民医院临床营养科,201306

出　　处：《医学研究杂志》2016年第3期18-21,25,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(面上项目)(81273061);黑龙江省教育厅科学技术研究项目(12531263)

摘　　要：目的观察γ-生育三烯酚(γ-T3)对转化生长因子(TGF-β1)诱导人胃癌SGC-7901细胞发生上皮间质转化(EMT)细胞迁移和侵袭能力的调控作用,探讨其可能的抑制机制。方法用浓度为10ng/ml TGF-β1和10ng/ml TGF-β1+30μmol/Lγ-T3分别作用于SGC-7901细胞24 h;利用倒置显微镜观察细胞形态学变化,并采用体外划痕实验、细胞侵袭实验和蛋白印迹等技术,检测γ-T3对TGF-β1诱导的胃癌细胞迁移和侵袭能力,以及E-cadherin和vimentin蛋白表达的影响。结果γ-T3抑制TGF-β1诱导人胃癌SGC-7901细胞发生EMT。10ng/ml TGF-β1组细胞多数呈梭型,细胞边界模糊,细胞间间隙大;10ng/ml TGF-β1+30μmol/Lγ-T3组细胞呈不规则多边形,细胞边界较清晰,间隙小。γ-T3抑制由TGF-β1诱导的SGA7901细胞的迁移能力(F=74.106,P<0.05)。γ-T3抑制由TGF-β1诱导的SGA7901细胞的侵袭能力(F=181.921,P=0.000)。γ-T3抑制TGF-β1诱导的E-cadherin蛋白表达降低(F=305.818,P=0.000);γ-T3也抑制TGF-β1诱导的vimentin蛋白表达增强(F=456.036,P=0.000)。结论γ-T3可能通过上调E-cadherin和下调vimentin蛋白表达,抑制TGF-β1诱导的人胃癌SGC-7901细胞发生EMT,降低胃癌细胞的迁移和侵袭能力。Objective To determine the effects of γ-tocotrienol （γ-T3） on the cell migration and invasion of cells and its possible inhibitory mechanism.Methods Transforming growth factor （TGF-β1） was used to induce human gastric cancer SGC -7901 cells in this study.SGC-7901 cells were treated with different concentrations of TGF-β1 or 10ng/ml of TGF-β1 with 30μmol/L of γ-T3 for 24h. The cellular morphological changes were observed under inverted microscope. The cell migration and invasion ability were detected by scratch wound assay and Transwell chamber in vitro invasion assay, respectively. The expression of E-cadherin and vimentin were also examined by western blotting. Results γ-T3 inhibited the epithelial-mesenchymal transition （EMT） of SGC-7901cells induced by TGF-β1. 10ng/ml of TGF-β1 induced morphological alteration from epithelial to mesenehymal cells i.e. the majority of cells turned to fusiform, fuzzy border. γ-T3 at dose of 30μmol/L also inhibited the EMT of SGC-7901 cells to show irregular polygon, clear border and small intercellular. γ-T3 inhibited the migration ability of SGC-7901 cells induced by TGF-β1. The cell number of TGF-β1 （10ng/ml） with γ-T3 （30μmol/L） group migrated to the scratch area was significantly less than that of only TGF-β1 （10ng/mL） group （F＝74.106, P〈0.05）. γ-T3 inhibited the invasion capacity of SGC-7901 cells induced by TGF-β1 （F＝181.921, P=0.000）. γ-T3 significantly inhibited the decreased expression of E-cadherin induced by TGF-β1. （F=305.818, P=0.000）. And γ-T3 also inhibited the increased expression of vimentin induced by TGF-β1 （F=456.036, P=0.000）. Conclusion The inhibitory effects of γ-T3 on EMT of SGC-7901 cells induced by TGF-β1 may affect expression of E-cadherin and vimentin protein to decrease the capacity of cell migration and invasion.

关 键 词：生育三烯酚 胃癌 上皮间质转化 

分 类 号：R5[医药卫生—内科学]