PDE5基因沉默对肾透明细胞癌细胞增殖和凋亡的影响  

Effects of PDE5 Gene Silence on the Proliferation and Apoptosis of Clear Cell Renal Cell Carcinoma Cells

作　　者：傅晶[1] 陈尧康[1] 虞利民[1] 何小祥[1] 黄正[1] 叶华建 

机构地区：[1]浙江大学医学院附属第一医院绍兴分院、绍兴市第二医院泌尿外科,312000

出　　处：《医学研究杂志》2016年第3期112-115,共4页Journal of Medical Research

基　　金：绍兴市科技计划项目(2010D10014)

摘　　要：目的探讨PDE5基因对肾透明细胞癌细胞株786-O细胞增殖和凋亡的影响。方法设计、合成PDE5 si RNA序列,按照Hi Per Fect转染试剂盒操作手册转染786-O细胞。采用实时定量聚合酶链反应(real-time quantitative PCR)和Western blot法检测转染后PDE5基因的表达,通过WST-1法检测细胞增殖情况,caspase-3活性检测试剂盒检测caspase-3活性。结果与空白对照组和阴性对照组相比较,PDE5 si RNA转染组的m RNA及蛋白表达水平显著降低,786-O细胞增殖能力明显受到抑制,caspase-3活性明显增加,差异均具有统计学意义(P<0.05)。结论 si RNA干扰PDE5基因表达可抑制肾透明细胞癌细胞株786-O细胞的增殖并促进凋亡。Objective To explore the effect of PDE5 gene on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O. Methods The siRNA of PDE5 was constructed and transfected into 786-O cells with HiPerFect transfection reagent. The PDE5 expression levels after transfection were detected by using real-time quantitative PCR and Western blot. The cell proliferation was assessed by WST-1 assay. The caspase-3 activity was detected by kit. Results Compared with the control group and blank group, the mRNA and protein levels were significantly reduced and the proliferation of 786-O cells was significantly inhibited in the PDE5 siRNA group （P〈0.05）, while caspase-3 activity was significantly increased （P〈0.05）. Conclusion siRNA interference of PDE5 gene may inhibit cell proliferation and promote cell apoptosis in clear cell renal cell carcinoma cell line 786-O.

关 键 词：肾透明细胞癌 PDE5基因 细胞增殖 细胞凋亡 

分 类 号：R3[医药卫生—基础医学]