大鹏澳不同区域尿素浓度与浮游植物脲酶活性的研究  

Study on urea concentration and urease activity of phytoplankton in different areas of Dapeng Cove

作　　者：韩婷婷[1] 齐占会[1] 戴明[1] 廖秀丽[1] 吴风霞[1] 巩秀玉[1] 付贵权[1] 黄洪辉[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东省渔业生态环境重点实验室,农业部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《南方水产科学》2016年第2期7-12,共6页South China Fisheries Science

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014A01YY03,2014TS01);广东省自然科学基金项目(2014A030310331);国家科技支撑计划项目(2011BAD13B02);农业部南海渔业资源开发利用重点实验室开放基金(LSF2014-05)

摘　　要：2014年8月对大鹏澳的核电温排区、人工鱼礁区和水产养殖区，以及连接大鹏澳的南涌河口、龙岐河口和王母河口等6个区域的尿素浓度（以氮浓度表示，Urea—N）和浮游植物脲酶活性开展调查研究，结合其他环境因子，对比分析不同区域的尿素分布特征及其与脲酶活性的相关性。结果表明，夏季大鹏澳湾内和河口Urea—N浓度相差较大，范围分别为0．28—1．21 μmol·L-1和0．38～3．50 μmol·L-1，相当于溶解无机氮（DIN）的6．30％-24．31％和3．13％～6．77％，氮源以DIN为主。龙岐河口和王母河口的尿素浓度最高，养殖区次之。湾内和河口的脲酶活性（以NH4-N表示）范围分别为0．61～1．03 μmol·（L·h）。和0．82～1．07 μmol·（L·h）-1，其活性除了与尿素浓度呈正相关外，还受无机营养盐和浮游植物量的影响。在氮、磷没有限制的鱼礁区、养殖区和南涌河口，叶绿素a（Chl-a）浓度高可促进脲酶的诱导调节。尿素为养殖区的重要氮源，脲酶活性与Chl-a呈极显著正相关。温排区、龙岐河口和王母河口存在磷限制，抑制脲酶的诱导调节。Based on the data of the cruise that was carried out in Dapeng Cove, mainly including thermal discharge area from nucle- ar power plant, artificial reef area, aquaculture area and three main estuarine areas into Dapeng Cove, Nanyong River, Longqi River and Wangmu River in Aug. , 2014 （summer） , we investigated the urea concentration （expressed by nitrogen, Urea-N） and urease activity of phytoplankton together with the other environmental parameters to discuss the bioavailability of urea for phytoplank- ton. Obvious difference between the bay and the estuary with average urea concentrations ranging from 0. 28 to 1.21 μmol.L-1 and 0. 38 to 3.50 μmol.L-1 , respectively, and the urea concentration was 6.30% -24. 31% of DIN in the bay and 3.13% -6. 77% of DIN in the estuary, resulting in inorganic nitrogen source for phytoplankton in Dapeng Cove. Urea concentration reached the maxi- mum at Longqi estuary and Wangmu estuary, followed by aquaculture area. The urease activity of phytoplankton in the bay and theestuary ranged from O. 61 to 1.03 μmol. （L.h） -1 and O. 82 to 1.07 μmol- （L.h） - 1, respectively. Urease activity was positively re- lated with urea concentration, and was also affected by inorganic nutrient and phytoplankton biomass. In tile artificial reef area, aq- uacuhure area and Nanyong estuary without N and P limitation, high Chl-a concentration could promote urease activity; in the aqua- culture area, urea was the main nitrogen source for phytoplankton, and Chl-a had very significant positive correlation with urease ac- tivity ; in the thermal discharge area （Longqi estuary and Wangmu estuary） , P limitation resulted in inhibitory regulation of urease activity.

关 键 词：大鹏澳 尿素 脲酶 夏季 相关性分析 

分 类 号：X834[环境科学与工程—环境工程]