不同发育阶段的四指马鲅消化道组织学比较研究  

Histology observation in digestive tract of Eleutherollema tetradactylum at different developmental stages

作　　者：谢木娇 区又君[1] 李加儿[1] 温久福[1] 王鹏飞[1] 王雯[1,2] 陈世喜[1,2] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《南方水产科学》2016年第2期51-58,共8页South China Fisheries Science

基　　金：广东省科技计划项目(2014A020217010);广东省海洋渔业科技与产业发展专项(A201401A05);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014YD04)

摘　　要：采用常规石蜡切片和H-E染色技术研究不同发育阶段的四指马鲅(Eleutheronema tetradactylum)消化道的结构特征。结果显示:1)35日龄与43日龄稚鱼体态相似,体表被覆鳞片,背鳍、胸鳍、臀鳍、尾鳍发育健全,前者全长(12.850±1.280)mm,后者(15.600±1.600)mm。65日龄幼鱼消化道整体依次分为食道、胃部和肠道,胃部又分贲门部、幽门部、胃体部和盲囊部,在幽门部连接肠道处分布4束短小分散成菜花状的幽门盲囊;肠道系数为0.630±0.002,分前、中、后肠。成鱼食道、胃部、肠道直径增大;肠道系数为0.300±0.010。2)两阶段的稚鱼消化道组织结构发育基本与幼鱼、成鱼相似,消化管壁由内至外依次为黏膜层、黏膜下层、肌肉层和外膜。消化管内黏膜皱褶、胃腺、黏膜上皮杯状细胞的数量在稚鱼、幼鱼、成鱼之间呈递增趋势,体现结构和功能逐渐发育完善的规律。The study investigates the construction of digest tract of Eleutheronema tetradactylum at different developmental stages by u- sing conventional paraffin section and H＆E staining. Results show that the body shapes of 35 dah and 43 dah juveniles were similar, with scales covering, dorsal fin, pectoral fin, anal fin and tail fin well constructed. The total lengths of former and the latter were （ 12. 850 ± 1. 280） mm and （ 15. 600 ± 1. 600） ram, respectively. The digest tract in 65 dah fish composted of esophagus, stomach and intestine ; the stomach consisted of cardiac stomach, pyloric stomach, fundic stomach and gastric caeca; four pyloric caeca scat- tered between pyloric and intestine ; intestinal coefficient was 0. 630 ± 0. 002 ; the intestine consisted of foregut, midgut and hindgut. The diameters of esophagus, stomach, intestine increased in adult fish with intestinal coefficient of 0. 300±0. 010. The histological a- nalysis reveals that the structures of digest tract in 35 dah and 43 dah juveniles were functionally similar to those of young and adult fish. The pipe wall consisted of mucosal, submucosal, muscular layer and serosa. The number of mucosal fold, gastric gland and goblet cell in the epithelium mucosal showed increasing regularity among juvenile, young fish and adult fish, which shows that the di- gestive tract developed gradually in structure and funtion.

关 键 词：四指马鲅 消化道 组织学 

分 类 号：S917.4[农业科学—水产科学]