傣药嘿良冲剂联合环磷酰胺对S180荷瘤模型小鼠增效减毒作用的研究  

Attenuation and Synergistic Effect of Heiliang Chongji Combined with Clophosphamid in S180 Tumor-Bearing Mice

作　　者：李俊[1] 史聪翀[2] 万春平[1] 祁燕[1] 郑喜[1] 李小丝[1] 李斯文[1] 

机构地区：[1]云南中医学院第一附属医院,云南昆明650021 [2]昆明医科大学附属口腔医院,云南昆明650021

出　　处：《中华中医药学刊》2016年第4期846-848,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81460624);云南省中医医院院内项目(2012YJ017)

摘　　要：研究傣药嘿良冲剂配合化疗药物治疗肿瘤的增效减毒作用及初步机制,为民族药嘿良冲剂的院内制剂的开发和临床应用奠定了理论基础。方法:建立S180荷瘤小鼠模型,随机分为荷瘤模型组、环磷酰胺(cyclophosphamid,CTX 30mg/kg)阳性组、嘿良冲剂(16 g/kg)组和嘿良冲剂联合CTX组,给药21后,分离肿瘤组织,称重并计算肿瘤抑制率;MTT法测定各组豆刀蛋白(Con A)诱导小鼠T淋巴细胞增殖功能;酶联免疫吸附法(ELISA)法检测丝裂原诱导脾淋巴细胞IFN-γ产生水平。结果:与单用环磷酰胺相比,嘿良龙冲剂联合环磷酰胺增加肿瘤抑制率,显著提高受抑制的T淋巴细胞功能的恢复,差异有显著性意义(P﹤0.01);嘿良冲剂联合环磷酰胺治疗显著上调Con A诱导T淋巴细胞IFN-γ的产生水平,差异有显著意义(P﹤0.05)。结论:嘿良龙冲剂联合环磷酰胺具有增效减毒效应,其初步机制可能与促进Th1细胞因子IFN-γ的产生,提高机体的抗肿瘤免疫功能有关。Objective： To investigate the attenuation and synergistic effect of Heiliang Chongji（ HLCJ） and chemotherapy and to provide a basis for clinical application of antitumor immunity and the development of hospital preparation.Method The S180 tumor-bearing mice were established to investigate the attenuation and synergistic effect of HLCJ.Mice were divided into four groups including model group,CTX group,HLCJ（ 16 g / kg,p.o.） groups and combination of HLCJ and CTX group.After 10 days of intragastric administration,the tumor tissues were isolated and weighted and the inhibited rates were calculated.The proliferation of splenocytes from different groups in response to Con A was determined by MTT method and the cytokine production levels of IFN-γ in Con A-induced splenic lymphocytes were detected by ELISA.Results： Compared to CTX group,the combination of HLCJ and CTX can significantly enhance the inhibited rate of tumor（ P〈0.05） and increase the proliferation activity in response to Con A（ P ﹤ 0.01）.The ELISA result showed that the production of IFN-γ in Con A-induced splenic lymphocytes was increased in combination of HLCJ and CTX group（ P ﹤ 0.05）.Conclusion： The results of this study indicated that HLCJ combined with CTX has the attenuation and synergistic effect on S180 tumor-bearing mice and the mechanism may be due to the increase of the secretion of IFN-γ and the improvement of antitumor immunity.

关 键 词：疫功能有关.关键词:嘿良冲剂 增效减毒作用 S180荷瘤小鼠 抗肿瘤免疫 IFN-γ 

分 类 号：R285.5[医药卫生—中药学]