柴芍承气汤对大鼠肠缺血再灌注后肝组织半胱氨酰白三烯受体1的表达和肝细胞凋亡的影响  

Effect of Chaishao Dachengqi Decoction on Cysteinyl Leukotriene Receptor 1 Expression of Intestinal Tissue in Rats with Intestinal Ischemia-Reperfusion and Apoptosis of Intestinal Mucosa

作　　者：吴深宝[1] 洪小飞[1] 朱旭星[1] 童秀萍[1] 王园园[1] 

机构地区：[1]温州医学院附属义乌医院消化科,浙江义乌322000

出　　处：《中华中医药学刊》2016年第4期956-959,I0008,I0009,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省医学会临床科研基金项目(2012ZYC-A87);义乌市科技局项目(13-3-17)

摘　　要：目的:研究中药柴芍承气汤对大鼠肠缺血再灌注(I/R)继发肝损伤的保护作用及其机制。方法:30只大鼠分为3组,对照组、模型组和中药组各10只。通过免疫组织化学染色方法、Western blot和RT-PCR法检测大鼠肝组织半胱胺酰白三烯受体1(cysteinyl leukotriene receptor 1,Cys LTR1)蛋白和mRNA表达水平,并用光学显微镜观察肝脏病理改变,TUNEL方法检测肝细胞凋亡。结果:(1)与对照组比较,模型组有大鼠肝脏病理损伤,中药组大鼠组肝脏组织的病理改变明显减轻(均P<0.05);(2)肠I/R时肝组织Cys LTR1蛋白和mRNA表达水平升高;应用中药后,肝组织Cys LTR1蛋白和mRNA表达水平均减低(均P<0.05);(3)与对照组比较,模型组肝细胞凋亡指数升高;与模型组比较,中药组肝细胞凋亡显著减少(P<0.01);(4)肝组织中Cys LTR1蛋白表达分别与细胞凋亡、肝脏组织病理损伤正相关(r=0.8463、0.8783、0.7708,均P<0.05)。结论:柴芍承气汤能减轻肠I/R继发肝脏损伤的病理损害和降低肝细胞凋亡,并能抑制肝脏组织中Cys LTR1的表达,柴芍承气汤可能通过抑制肝脏组织中Cys LTR1的表达和抑制肝细胞凋亡减轻肠I/R继发肝损伤。Objective： To study the protection mechanism of Chaishao Chengqi Decoction on rats with intestinal ischemia-reperfusion（ I / R）.Methods： 30 rats were divided into 3 groups： sham operation group,I / R model group and treatment group with 10 rats in each group.The expression of Cys LTR1 protein and mRNA levels in intestinal tissue were detected by immunohistochemical staining,Western blot and RT-PCR and the optical microscope was used to detect the pathological changes of small intestine.The small intestine weight change was detected.Furthermore,the apoptosis of intestinal mucosa was detected with TUNEL method.Results：（ 1） Compared with the control group,pathological changes of intestinal tissue in rats with traditional Chinese medicine treatment were reduced significantly（ P〈0.05）.Intestinal water content in model group was significantly higher than that in the control group and traditional Chinese medicine group（ P〈0.01）.（ 2） The expression levels of Cys LTR1 protein and mRNA were increased in intestinal tissue of I/R rats.Traditional Chinese medicine could reduce the expression levels of Cys LTR1 protein and mRNA in intestinal tissue.（ 3）Compared with the model group,apoptosis index of intestinal mucosa cells in Chinese medicine group was significantly decreased（ P〈0.01）.（ 4） The expression of Cys LTR1 protein in the intestinal mucosa was correlated with cell apoptosis,pathological damage of mucosal injury and intestinal water content（ R values were respectively 0.8463,0.8783,0.7708,P〈0.05）.Conclusion： Chaishao Chengqi Decoction could release the pathological change of intestinal tissue and could decrease the apoptosis of intestinal mucosa cells and inhibit the expression of Cys LTR1 of intestinal mucosa in rats.It suggested that Chaishao Chengqi Decoction protect intestinal mucosal injury by inhibiting Cys LTR1 expression.

关 键 词：大鼠 肠缺血再灌注 肝脏病理 柴芍承气汤 胱氨酰白三烯受体1 凋亡 

分 类 号：R258.8[医药卫生—中医内科学]