优化制备生物素化超声微泡的实验研究  

Optimizing Preparation of Biotin-microbubbles of Ultrasound

作　　者：杨阳[1] 朱梅[1] 刘政[2] 章晓锋 张海琳[1] 冯煜然 李抒[3] 

机构地区：[1]昆明医科大学第一附属医院超声科,云南省超声影像研究中心,云南昆明650031 [2]第三军医大学新桥医院超声科,重庆400037 [3]云南省药物依赖防治研究所,云南昆明650228

出　　处：《昆明医科大学学报》2016年第1期19-23,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81160178);云南省中青年学术和技术带头人后备人才项目(2014HB029)

摘　　要：目的优化制备粒径小、稳定性好的靶向微泡前体-生物素化微泡,并评价其理化性质及显影效果.方法选用不同膜材料配比制备生物素化脂质微泡,进行分组（A-E组）对照研究,粒径分析仪、Malvern表面电位仪评价各组理化性质;选用不同溶酶液,分组（1~3组）评价对微泡稳定性的差异;超声诊断仪下观察各组生物素化微泡的显影效果.结果在A-E组中,A、C、E 3组有较明显的差异（P〈0.05）：（1）西林瓶中可见脂质呈不同分层状态;（2）随DPPC的摩尔比不断增加,MPEG200、DPPA摩尔比不断减小,微泡的平均粒径逐渐减小,粒径分布逐渐均一,浓度逐渐增高,Zeta电位逐渐变小且更加稳定;（3）使用三羟甲基氨基甲烷,甘油,丙二醇复合溶酶液制备的微泡微相互融合慢、粒径变化小、破裂少、浓度降低少.结论按DPPC：MPEG200：DPPA：DSPE-PEG2000-Biotin 90：5：5：10且选用复合溶酶液优化制备的生物素化脂质微泡是最佳的淋巴系统靶向脂质微泡制作前体.Objective To optimically prepare for biotin-microbubbles with small diameter and good stability,and evaluate the physical and chemical properties,and the effect of contrast.Methods We prepare lipid vesicles with different proportion for the group A-E.The physical and chemical properties of each group were evaluated by particle size analyzer and Malvern.We evaluated the effect of different solvents on the stability of the group1-3,observed the effect of each group by ultrasonic diagnostic apparatus.Results There were significant differences between groups A,Cand E：Lipid located the different layers of the vial;With the increasing of DPPC,MPEG200,DPPA,the average size and concentration decreased gradually,the distribution became uniform gradually,and the Zeta decreased gradually.Complex solution made microbubbles with small size, non-rupture, concentration maintain and good stability.Conclusions DPPC：MPEG200：DPPA：DSPE-PEG2000-Biotin 90：5：5：10 and complex solution can make the best biotin-microbubbles.It is a optimal choice for preparation of target microbubbles.

关 键 词：生物素 亲和素 微泡 制备 显影 

分 类 号：R445.1[医药卫生—影像医学与核医学]