富血小板血浆联合人脐带间充质干细胞移植对大鼠骨质疏松骨折愈合的影响  

Effect of platelet-rich plasma combined with human umbilical cord-mesenchymal stem cells on the healing of osteoporotic fracture in rats

作　　者：楚佳奇 孙杰聪[1] 李鹏[2] 李广盛[1] 牛艳茹[1] 林颢[1] 陈光华[1] 魏波[1] 魏劲松[1] 曾荣[1] 

机构地区：[1]广东医学院附属医院骨科中心,湛江524001 [2]广东医学院附属医院干细胞研发与细胞治疗中心,湛江524001

出　　处：《中国骨质疏松杂志》2016年第3期283-287,共5页Chinese Journal of Osteoporosis

基　　金：广东省自然科学基金项目(S2013020012866);广东省医学科研基金项目(B2014313);湛江市科技计划项目(2014A06005);广东医学院科研基金项目(Z2013005)

摘　　要：目的探讨PRP联合h UC-MSCs移植对大鼠骨质疏松性骨折愈合的影响。方法雌性SPF级SD大鼠50只随机分为5组,每组10只:假手术组(仅切除卵巢周围组织),安慰剂组(注射生理盐水),未诱导组(给予未诱导的h UC-MSCs),成骨诱导组(移植诱导后的h UC-MSCs),PRP+成骨诱导组(移植诱导后的h UC-MSCs联合PRP)。OPF造模成功后,对各组动物进行细胞移植3次,分别在OPF造模后1周、3周和7周。骨折后的第1周、3周、5周、7周、11周、13周Micro CT扫描动物模型患肢。结果 Micro CT动态观察结果显示,PRP+成骨诱导组骨痂生成量明显多于、早于其余各组,骨折11周时达到临床愈合标准,而其他组别骨痂重塑期相对滞后,比正常骨量大鼠愈合时间至少推迟了37.5%。结论 PRP联合h UC-MSCs移植可促进大鼠骨质疏松性骨折愈合,为临床治疗OPF提供数据支持。Objective To investigate the effect of PRP combined with h UC-MSCs transplantation on the healing of osteoporotic fracture in rats.Me thods Fifty female SPF grade SD rats were randomly divided into 5 groups,10 rats in each group：The sham operation group was only resected ovarian tissues;The placebo group was injected with saline;The non-induction group transplanted withuninducedh UC-MSCs;The PRP ＋ induction group transplanted withh UC-MSCs combined with PRP.After the OPF model was successfully constructed,cells were transplanted 3 times in each group,1 week,3 weeks and 7 weeks after fracture.Micro-CT analysis was performed every two weeks after fracture.Re sults Micro-CT analysis results showed that the amount of callus formation in PRP ＋ induction group was significantly increased than that in the other groups.Clinicalhealing criteria was achieved at11 weeks post-fracture,while other groups callus remodeling phase is lagging behind.The bone healing time in rats was delayed for at least 37.5% compared with normal fracture animals.Conclusion PRP combined with h UC-MSCs transplantation promote osteoporotic fracture healing in rats.

关 键 词：富血小板血浆 脐带间充质干细胞 成骨分化 骨质疏松性骨折 

分 类 号：Q813.3[生物学—生物工程]