电针复合七氟烷麻醉对断尾大鼠急性应激反应调控的影响  

Effect of electro-acupuncture combined with sevoflurane anesthesia on regulation of acute stress response in docked tails rats

作　　者：胡越[1] 刘莉 熊威威[3] 谭毅[3] 黄志华[3] 李爱国[3] 杨华[4] 蒋奕红[3] 

机构地区：[1]桂林医学院研究生院,广西桂林541001 [2]湖北省武汉市第三医院麻醉科,430000 [3]桂林医学院附属医院麻醉科,广西桂林541001 [4]桂林医学院附属医院中医科,广西桂林541001

出　　处：《重庆医学》2016年第10期1306-1308,1312,共4页Chongqing medicine

基　　金：2013年广西中医药民族医药继承创新工程(GZZC13-20)

摘　　要：目的探讨不同穴位电针刺激复合七氟烷麻醉对断尾大鼠急性应激反应调控作用的影响。方法健康成年雄性SD大鼠50只,体质量280~320g,分为正常组（Ⅰ组）、模型组（Ⅱ组）、七氟烷吸入组（Ⅲ组）、七氟烷吸入加体针组（Ⅳ组）和七氟烷吸入加耳针组（Ⅴ组）,每组10只。Ⅰ组不作处理置于氧流量30%的混合气体玻璃箱中（流量2L/min）,Ⅱ组断尾后置于氧流量30%的混合气体玻璃箱中（流量2L/min）,Ⅲ、Ⅳ、Ⅴ组断尾后置于含体积分数为2%的七氟烷麻醉箱中（流量2L/min）,Ⅳ组持续刺激双侧＂足三里＂穴30min（刺激参数3V,3Hz,2ms）,共4次,每次间隔30min,Ⅴ组刺激双侧＂耳尖＂穴,刺激参数及时间同Ⅳ组。各组治疗方法结束后,处死各组大鼠,取断颈血采用ELISA测促肾上腺皮质激素（ACTH）和外周血皮质酮（CORT）水平。采用Western blot法测定下丘脑组织c-fos蛋白的表达水平。结果与Ⅰ组相比,Ⅱ组血清ACTH、CORT水平、下丘脑c-fos蛋白表达明显升高（P〈0.01）;与Ⅱ组相比,Ⅲ、Ⅳ、Ⅴ组大鼠外周血ACTH、CORT水平均有所下降,差异有统计学意义（P〈0.05）;Ⅲ组与Ⅳ组的ACTH和CORT水平差异无统计学意义（P〉0.05）;Ⅴ组的ACTH水平与Ⅲ组相比明显下降（P〈0.05）,Ⅴ组CORT水平与Ⅳ组相比明显下降（P〈0.05）。Ⅲ、Ⅳ、Ⅴ组c-fos表达与Ⅱ组相比均降低（P〈0.05）,Ⅲ、Ⅳ、Ⅴ组3组组间差异有统计学意义（P〈0.05）。结论七氟烷吸入复合电刺激＂耳尖＂穴治疗时,对应激的作用优于单纯的七氟烷治疗和七氟烷复合电刺激＂足三里＂穴。电针复合吸入全身麻醉对应激的调控机制可能与下丘脑调节室旁核c-fos蛋白分泌并影响下丘脑-垂体-肾上腺（HPA）轴有关。Objective To investigate the effect of electro-acupuncture combined with sevoflurane anesthesia on the regulation of acute stress response in docked tails rats.Methods Fifty male SD rats weighing 280-320 g were randomlsy divided into 5groups（n=10each）：normal group（groupⅠ）,model group（groupⅡ）,sevoflurane group（groupⅢ）,sevoflurane inhalation combined with body acupuncture group（group Ⅳ）and sevoflurane combined with auricular acupuncture group（groupⅤ）.The groupⅠwas placed in a glass box containing mixture gas with oxygenflow of 30%（flow 2L/min）without treatment,the group Ⅱafter docking tail was placed in a glass box containing mixture gas with oxygenflow of 30%（flow 2L/min）,the group Ⅲ,Ⅳ and Ⅴwere placed in the 2%sevoflurane anesthesia box（flow 2L/min）,the groupⅣ was given continuous stimulation at Zusanli（stimulation parameters：3V,3Hz,2ms）for 30 min per time,4times at intervals of 30 min,the groupⅤ was given the stimulation at Erjian with the same stimulation parameters as the groupⅣ.Rats in each group were killed after treatment and the blood at broken neck was collected for measuring serum corticosterone（CORT）and adrenocorticotropic hormone（ACTH）levels by ELISA.The cfos protein expression level in hypothalamic tissue was detected by Western blot.Results Compared with the group Ⅰ,serum ACTH,CORT levels,hypothalamic expression of c-fos protein in the group Ⅱwere significantly increased（P〈0.01）,compared with the group Ⅱ,the peripheral blood ACTH,CORT levels were decreased with statistical difference（P〉0.05）;the ACTH,CORT levels had no statistical difference between the groupⅢ andⅣ（P〈0.05）;the ACTH level in the groupⅤ was significantly decreased compared with the groupⅢ（P〈0.05）and the CORT level in the group Ⅴ was also decreased when compared with the group Ⅳ（P〈0.05）.The c-fos expression in the groupⅢ,Ⅳ and Ⅴwere decreased compared with the groupⅡ（P〈0.05）,there were significant d

关 键 词：应激 电针 原癌基因蛋白质C-FOS 七氟烷 耳针 

分 类 号：R245[医药卫生—针灸推拿学]