脊髓损伤犬模型的建立及激光多普勒血流测定仪评价的意义  

Establishment of canine spinal cord injury model and significance of laser-doppler flowmetry evaluation

作　　者：武玉江[1] 

机构地区：[1]河北省邢台市人民医院脊柱骨科,054001

出　　处：《重庆医学》2016年第10期1320-1322,1325,共4页Chongqing medicine

摘　　要：目的探讨激光多普勒血流测定仪(LDF)在建立的急性犬脊髓损伤模型中的评价作用。方法将15只比格犬分为A、B、C组。通过改良的脊髓损伤数控装置,采用不同的撞击和压迫时间,建立不同的急性脊髓损伤犬模型。A组为1.2m/s+5mm+5mm+15mns,B组为1.2m/s+5mm+5mm+10mns,C组为对照组。应用LDF分别测量3组脊髓损伤模型的脊髓血流(SCBF)图,通过脊髓坏死面积测量评估两组模型犬的病理学改变。结果 LDF测量3组脊髓损伤模型的SCBF图结果显示,A组模型SCBF量降低最明显,A组损伤程度明显重于B、C组,差异有统计学意义(P<0.05)。运动诱发电位显示,SCBF的下降程度与预后及运动功能密切相关。结论通过LDF可有效判断急性脊髓损伤的程度和SCBF的情况,在动物实验和临床领域有较好的应用前景。Objective To investigate the effect of laser-doppler flowmetry（LDF）in the evaluation of established canine spinal cord injury（SCI）model.Methods Totally 15 beagle dogs were randomly divided into the group A,B and C.Through the improved numerical control SCI apparatus,different impacts and compression time were adopted to establish the different acute SCI models.The group A was 1.2m/s＋5mm＋5mm＋15mns,the group B was 1.2m/s＋5mm＋5mm＋10mns,while the group C was taken as the control group.LDF was used to measure the spinal cord blood flow（SCBF）chart,the pathologic change was evaluated by measuring the spinal cord necrosis area.Results The SCBF chart in 3groups measured by LDF showed that the decrease of SCBF in the group A was most obvious,the injury degree in the group A was more severe than those in the group B and C,the difference was statistically significant（P〈0.05）.The motor evoked potential displayed that the decrease degree of SCBF was closely related with the prognosis and motion function.Conclusion LDF could effectively judge the degree of spinal injury and SCBF situation,which has a good application prospect in animal experiments and clinical field.

关 键 词：激光多普勒流量测定 脊髓损伤 血流动力学 犬模型 

分 类 号：R651.2[医药卫生—外科学] R-332[医药卫生—临床医学]