黄芪注射液通过调节JNK和NF-κB通路抑制视网膜神经节细胞凋亡  

Astragalus Injection Inhibits Retinal Ganglion Cell Apoptosis by Regulating JNK and NF-κB Pathway

作　　者：谷新怡 周剑[2] 闫晓玲[2] 苏艳[2] 吴鲁华[3] 赵朋波 刘昕妍[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078 [3]北京中医药大学第三附属医院,北京100029

出　　处：《中国中医基础医学杂志》2016年第3期358-361,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81173307)-补气活血法对视神经p75NTR/TrK信号通路调控的分子机制研究

摘　　要：目的:观察黄芪注射液对视神经牵拉伤大鼠视神经保护作用并探讨其作用机制。方法:将66只SPF级健康Wistar雄性大鼠按随机数字表法分为假手术组、视神经牵拉伤模型组及黄芪注射液治疗组3组各22只大鼠;横向定量牵拉法制作大鼠视神经损伤模型,假手术组仅暴露视神经不予牵拉;术后治疗组给予黄芪注射液腹腔注射,模型组给予生理盐水腹腔注射;治疗14 d后取大鼠视网膜组织,利用蛋白免疫印迹法检测JNK和NF-κB表达变化,并采用real time-PCR方法检测p75NTR的mRNA水平。结果:与假手术组比较,视神经牵拉伤模型大鼠视网膜组织p75NTR mRNA水平及JNK蛋白表达明显上升,NF-κB蛋白表达显著下降;给药14 d后与模型组比较,黄芪注射液治疗组大鼠视网膜组织p75NTR mRNA水平及JNK蛋白表达显著减少,NF-κB蛋白表达明显升高。结论:黄芪注射液具有视神经保护作用,其机制可能与抑制p75NTR mRNA的表达及JNK蛋白水平、促进NF-κB的蛋白表达有关。Objective： To observe the optic nerve protective effects of astragalus membranaceus injection on tractive optic nerve injury in rats and to explore its possible mechanism. Methods： 66 healthy male Wistar rats were randomly divided into sham operated group, model group and astragalus membranaceus injection group, with 22 rats in each group; The optic nerve of model rats was injured by the transverse quantitative traction （ tension given to the optic nerve ： 0. 10N ; tractive direction： perpendicular to the optic nerve; duration： 20 seconds）, and that of sham-operation group was only exposed but not pulled; After model establishment, treatment group were given with astragalus membranaceus injection by intraperitoneal injection, and model group were given with normal saline for testing. 14 days later, the changes of expression of JNK and NF-gB in rat retinal tissue were detected with Western blotting, and the expression of p75NTR mRNA was detected by real-time PCR. Resuhs： Compared with the sham operation group, the expression level of p75NTR mRNA and JNK protein in the optic nerve injury group increased. On the contrary, the protein expression level of NF-KB significantly declined; After treatment for 14 days, the expression of p75NTR mRNA and JNK protein decreased in the astragalus injection treatment group compared with the model group. However, the protein expression level of NF-kB increased. Conclusion： Astragalus membranaceus injection could has the function of protecting the optic nerve. The mechanism may be related with decreasing p75NTR mRNA and the protein expression of JNK, and increasing the protein expression of NF-KB.

关 键 词：视神经损伤 黄芪 P75神经营养因子受体 C-JUN氨基末端激酶 核因子KB 

分 类 号：R285.5[医药卫生—中药学]