芥蓝花薹中莱菔硫烷含量的HPLC分析  

Analysis of Sulforaphane in Chinese kale(Brassica albograbra L. H. Bailey)by HPLC

作　　者：李占省[1] 刘玉梅[1] 方智远[1] 杨丽梅[1] 庄木[1] 张扬勇[1] 李凌云 孙培田[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]农业部蔬菜品质监测检验中心,北京100081

出　　处：《中国蔬菜》2016年第4期53-57,共5页China Vegetables

基　　金：国家自然科学基金项目(31372067;31501761);现代农业产业技术体系建设专项(CARS-25-A);国家科技支撑计划项目(2013BAD01B04);农业部园艺作物生物学与种质创制重点实验室项目;中国农业科学院科技创新工程项目(CAAS-ASTIPIVFCAAS)

摘　　要：以23份芥蓝纯合自交系和21份杂交F_1为试材,采用反相高效液相色谱法(RP-HPLC)测定花薹中莱菔硫烷含量,分析芥蓝花薹中莱菔硫烷含量的变化规律。结果表明:44份芥蓝材料花薹中莱菔硫烷含量变异范围为46.89~419.45mg·kg^(-1)(DW),供试材料间莱菔硫烷含量差异达显著水平。获得了1份莱菔硫烷含量较高的F_1材料A86,含量为419.45mg·kg^(-1)(DW);3份莱菔硫烷含量较高的高代纯合自交系材料A1、A17和A53,含量分别为298.50、354.19、361.06mg·kg^(-1)(DW),可用于芥蓝抗癌新品种的选育和利用。Forty four lines of Chinese kale（Brassica albograbra L.H.Bailey）including 23 high inbred lines and 21 F1 generations were used to detect the sulforaphane contents in flower stalk of Chinese kale by RP-HPLC,and to analyze the changing rule in sulforaphane contents.The results showed that the changing range of sulforaphane contents in 44 Chinese kale was 46.89-419.45 mg·kg-1（DW）,and there were significant differences between sulforaphane contents of the testing lines.Four lines were detected with higher sulforaphane contents,among them one line was F1 generation A86 with 419.45 mg·kg-1（DW）sulforaphane content,the other 3 were inbred lines A1,A17 and A53.Their sulforaphane contents were 298.50 mg·kg-1（DW）,354.19 mg·kg-1（DW）,361.06 mg·kg-1（DW）,respectively.They could be used for breeding of Chinese kale in anti-cancer and marketing promotion.

关 键 词：芥蓝 花薹 莱菔硫烷 高效液相色谱法 

分 类 号：S635.9[农业科学—蔬菜学]