补肾生髓方和益气活血方对局灶性脑缺血再灌注大鼠额顶叶皮质Notch信号通路Nurr1、SMO mRNA及其蛋白表达的影响  

Effects of Bushen Shengsui Prescription and Yiqi Huoxue Prescription on mRNA and Protein Expression of Nurr1 and SMO in Notch Signaling Pathway in Frontal and Parietal Cortex of Rats with Focal Cerebral Ischemia-Reperfusion

作　　者：宋祖荣[1] 徐伟[2] 王键[2] 胡建鹏[2] 

机构地区：[1]安徽新华学院药学院,安徽合肥230088 [2]安徽中医药大学新安医学教育部重点实验室,安徽合肥230038

出　　处：《安徽中医药大学学报》2016年第2期57-60,共4页Journal of Anhui University of Chinese Medicine

基　　金：国家科技支撑计划项目(2012BAI26B01);国家自然科学基金项目(30973692);安徽省教育厅自然科学研究重点项目(KJ2014A098)

摘　　要：目的探讨补肾生髓方和益气活血方对脑缺血再灌注大鼠额顶叶皮质Notch信号转导通路Nurr1、SMO mRNA及其蛋白表达的影响。方法采用线栓法复制右侧大脑中动脉阻塞局灶性脑缺血再灌注大鼠模型,将大鼠随机分为假手术组、模型组、补肾生髓方组和益气活血方组。脑缺血2h后,持续灌注7d。采用PCR检测额顶叶皮质Nurr1、SMO mRNA表达水平,采用Western blot检测其蛋白表达水平。结果与假手术组比较,模型组Nurr1、SMO mRNA及其蛋白表达水平均明显上调(P<0.05);益气活血方组和补肾生髓方组Nurr1mRNA及其蛋白、SMO蛋白相对表达水平均较模型组明显下调(P<0.05);模型组、益气活血方组和补肾生髓方组SMO mRNA相对表达水平比较,差异无统计学意义(P>0.05);益气活血方组与补肾生髓方组Nurr1、SMO蛋白表达水平有显著差异(P<0.05)。结论补肾生髓方和益气活血方促进脑组织修复的作用与其下调Notch信号转导通路中Nurr1、SMO mRNA及其蛋白表达有关。Objective To investigate the effects of Bushen Shengsui Prescription （for tonifying the kidney to regenerate bone marrow） and Yiq i Huoxue Prescription （for tonifying qi and activating blood circula- tion） on the mRNA and protein expression of Nurrl and SMO in the Notch signaling pathway in the frontal and parietal cortex of rats with focal cerebral ischemia-reperfusion. Methods A rat model of right mid-dle cerebral artery occlusion and focal cerebral ischemia-reperfusion was established by suture method. The rats were randomly divided into sham-operation group, model group, Bushen Shengsui group, and Yiqi Huoxue group. The perfusion started at 2 hours after cerebral ischemia and lasted for 7 days. PCR and Western blot were used to measure the mRNA and protein expression of Nurrl and SMO, respectively. Results Compared with the sham-operation group, the model group had significantly upregulated mRNA and protein expression levels of Nurrl and SMO （P〈0.05）. Compared with the model group, the Yiqi Huoxue group and Bushen Shengsui group had significantly down-regulated mRNA and protein expression levels of Nurrl and protein expression of SMO （P〈0.05）. The mRNA expression of SMO showed no significant differences between the model group, Yiqi Huoxue group, and Bushen Shengsui group （P〉0.05）. The protein expression levels of Nurrl and SMO showed significant differences between the Yiqi Huoxue group and Bushen Shengsui group （P〈0.05）. Conclusion Bushen Shengsui Prescription and Yiqi Huoxue Prescription can promote the repair of injured cerebral tissues, which is associated with its ability to down-regulate the mRNA and protein expression of Nurrl and SMO in the Notch signaling pathway.

关 键 词：脑缺血再灌注 益气活血方 补肾生髓方 Notch信号转导通路 

分 类 号：R285.5[医药卫生—中药学]