用于糖链抗原CA19-9实时动态活检的免疫双光子荧光标记试剂盒  

Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9

作　　者：黄池宝[1,2] 张道海[3] 曾伯平[1] 刘其斌[3] 陈华仕[1] 康帅[1] 陈晓远[2] 

机构地区：[1]遵义师范学院化学化工学院,遵义563002 [2]韶关大学农业科学与工程学院,韶关512005 [3]贵州大学材料与冶金学院,贵阳550025

出　　处：《高等学校化学学报》2016年第4期638-642,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21562050;11464052);贵州省第八批科技创新人才团队建设专项基金[批准号:黔科合人才团队(2015)4007];贵州省科学技术基金项目(批准号:黔科合J字[2015]2146;[2012]2345);贵州省教育厅自然科学重点研究项目(批准号:黔教合KY字[2014]296;[2013]171);贵州省教育厅省级本科教学工程建设项目(批准号:黔教[2014]JXGChcb);遵义市“15851人才精英工程”项目[批准号:(2015)4007];遵义市红花岗区科学技术项目(批准号:遵红科合社字[2015]18)资助~~

摘　　要：采用双光子荧光标记探针LP制备了免疫双光子荧光抗体LP-Ab,以特异性识别肿瘤标志物CA19-9(Ag)形成抗原-抗体复合物(LP-Ab·Ag),基于双光子诱导荧光机制用近红外激光(740 nm)激发LP-Ab·Ag,利用LP-Ab·Ag的双光子荧光实现了CA19-9的高清晰度、高灵敏度及高分辨率的量化检测和实时动态成像,并制成用于糖链抗原CA19-9检测的简单便捷的双光子荧光标记试剂盒.Carbohydrate antigen CA19-9（ Ag） is a tumor marker associated with pancreatic cancer,gallbladder cancer,colon cancer and gastric cancer in early stage of tumor and cancer stage. Antibody Ab19-9（ Ab）to carbohydrate antigens were labeled to form probe-labeled antibody LP-Ab using the prepared two-photon fluorescence probe LP. On the basis of the antigen-antibody specificity affinity principle,LP-Ab could combine with carbohydrate antigen Ag into a complex LP-Ab·Ag,which was excited by near infrared laser（ 740nm） and emitted strong two-photon fluorescence to accomplish the high sensitive biopsy and real-time dynamic high-resolution imaging of the tumor marker Ag with two-photon fluorescence microscope. Based on this,a simple and convenient two-photon fluorescent labeling kit was prepared to realize the rapid detection of tumor,which is convenient for large-scale clinic testing and screening. The research further broadens the application of LP,and provides a sharp tool for revealing the mechanism of cancer occurrence,development and metastasis. LP-Ab can be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.

关 键 词：糖链抗原CA19-9 抗CA19-9单克隆抗体(Ab19-9) 免疫荧光标记 双光子 活检 

分 类 号：O657[理学—分析化学]