IFAS中硝化细菌群落结构的演替与分析  

Succession and Analysis of Community Structure of Nitrifying Bacteria in Integrated Fixed-film Activated Sludge System

作　　者：李超[1,2] 田刚[3] 

机构地区：[1]天津生态城环境技术咨询有限公司 [2]天津大学环境科学与工程学院 [3]中国人民解放军空军93756部队

出　　处：《中国给水排水》2016年第7期48-52,共5页China Water & Wastewater

基　　金：天津市科技计划项目(14ZXCFSF00131)

摘　　要：为了揭示复合生物反应器(IFAS)中氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)群落结构的演替过程,利用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)和克隆测序等分子生物学技术对IFAS中AOB、NOB群落的演替进行了研究。DGGE结果表明,在反应器运行过程中,悬浮污泥和生物膜上的优势AOB和NOB种群均有较大区别,但是优势种群在系统中保持稳定,受水质波动影响较小。测序结果表明,悬浮污泥和生物膜中AOB的优势菌群分别为Nitrosospira sp.和Nitrosomonas sp.、β-Proteobacterium clone 4-70;NOB中的优势菌群分别为Nitrite-oxidizing bacterium MPN2、Pseudomonas sp.、Nitrospira sp.clone Nsp24和Nitrobacter sp.clone VAS9。To reveal the succession of community structure of ammonia-oxidizing bacteria （AOB） and nitrite-oxidizing bacteria （NOB） in the integrated fixed-film activated sludge （IFAS） system, the molecular biological techniques of polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）, cloning and sequencing were applied. The DGGE results showed that in the process of the experiment, the AOB and NOB in suspended sludge and biofilm were both quite different, and the dominant species were stable and less affected by fluctuation of water quality. The results of cloning and sequencing revealed that the dominant species in AOB were Nitrosospira sp., Nitrosomonas sp. and β- Proteobacterium clone 4-70 respectively, and the dominant species in NOB were nitrite-oxidizing bacterium MPN2, Pseudomonas sp., Nitrospira sp. clone Nsp24 and Nitrobacter sp. clone VAS9 respectively.

关 键 词：复合反应器 氨氧化菌 亚硝酸盐氧化菌 PCR—DGGE 

分 类 号：X703[环境科学与工程—环境工程]