碳氮比对生物膜SND过程微生物菌群结构的影响  

Effect of C/N Ratio on Microbial Community Structure in Biofilm during Simultaneous Nitrification and Denitrification

作　　者：罗菁蕾 张朝升[1] 荣宏伟[1] 

机构地区：[1]广州大学土木工程学院,广东广州510006

出　　处：《中国给水排水》2016年第7期96-100,共5页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51278133;21477027);广东省科技计划项目(2014A020216048);广东省自然科学基金资助项目(S2013010013943);广州市教育系统创新团队资助项目(13C01)

摘　　要：将传统水质检测方法和荧光定量PCR技术相结合,以序批式生物膜反应器(SBBR)为研究对象,选取总细菌、氨氧化菌(AOB)、硝化菌(NOB)和反硝化菌4种目的基因,对各菌群的特征形态进行表达,分析在不同碳氮比条件下生物膜不同微区各功能菌群的结构变化。结果发现,碳氮比对氨氮和总氮去除效果有明显影响。水质分析结果表明,当C/N值分别为4、6、8、10、12时,对氨氮的平均去除率分别为73.64%、77.85%、87.27%、90.20%、85.42%,对总氮的平均去除率分别为47.96%、57.34%、81.26%、87.03%、75.76%。荧光定量PCR结果表明,当C/N值分别为8、10、12时,AOB的平均拷贝数分别为2.50×107、3.08×107、7.85×106copies/ng;NOB的平均拷贝数分别为2.17×105、4.19×105、1.48×105copies/ng。由水质检测和定量PCR综合分析,当碳氮比为10时SBBR的脱氮效果最佳。The traditional water quality detection method was combined with the techniques of flu- orescent quantitative PCR, and the sequencing biofilm batch reactor （SBBR） was used to characterize the morphologies of total bacteria, ammonia-oxidizing bacteria （ AOB）, nitrite-oxidizing bacteria （NOB） and denitrifying bacteria. The changes of bacterial community structures in different micro-zones of the biofilm under different C/N ratios were analyzed. The results showed that the C/N ratio had significant effect on the removal of NH4^＋ - N and TN. From the water quality analysis, when the C/N ratio was 4, 6, 8, 10 and 12 respectively, the average removal rate of ammonia nitrogen was 73.64%, 77.85%, 87.27%, 90.20% and 85.42% respectively, and the average removal rate of TN was 47.96% , 57.34%, 81.26%, 87.03% and 75.76% respectively. From the fluorescence quantitative PCR analysis, when the C/N ratio was 8, 10 and 12 respectively, the average copy number of AOB was 2.50 × 10^7 copies/ng, 3.08 × 10^7 copies/ng and 7.85 × 10^6 copies/ng respectively, and the average copy number of NOB was 2.17 × 10^5 copies/ng, 4.19 × 10^5 copies/ng and 1.48 × 10^5 eopies/ng respectively. From the comprehensive analysis of water quality and fluorescent quantitative PCR, the treatment effect in SBBRwas the best when the C/N ratio was 10.

关 键 词：碳氮比 同步硝化反硝化 氨氧化菌 硝化菌 反硝化菌 荧光定量PCR 

分 类 号：X703[环境科学与工程—环境工程]