山蛩提取物对小鼠H_(22)移植瘤抑制作用研究  

Inhibitory Effect of Extracts from Orthomorpha Against H_(22) Tumor in Mice

作　　者：熊焱强[1,2] 孙耀华[1] 程雪[1] 李志红[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学仁和医院,湖北宜昌443002

出　　处：《亚太传统医药》2016年第7期5-7,共3页Asia-Pacific Traditional Medicine

基　　金：湖北省高等学校2014年大学生创新创业训练计划项目(201411075024);宜昌市科学技术研究与开发项目(A09302-11);三峡大学博士科技启动基金(KJ2008B054)

摘　　要：目的:研究山蛩提取物在小鼠体内对肝癌H_(22)移植瘤生长的影响。方法:肝癌H_(22)荷瘤小鼠随机分为5组:模型组、山蛩提取物低、中、高(0.40、0.80、1.60g·kg^(-1))3个剂量组和阳性药五氟尿嘧啶(5-FU,5mg·kg^(-1))对照组。连续给药13天,于第14天检测抑瘤率、脾指数、胸腺指数、血清中AST和ALT的活性。结果:与模型组比较,山蛩提取物低、中、高剂量组的抑瘤率分别为27.14%(P<0.05)、35.31%(P<0.01)和40.25%(P<0.01);同阳性组相比,山蛩提取物高、中、低剂量组的荷瘤小鼠脾脏指数及胸腺指数均显著升高(P<0.05)。结论:山蛩提取物对肝癌H_(22)荷瘤小鼠肿瘤的生长具有明显的抑制作用,并能保护荷瘤小鼠的免疫功能,其机制可能与其促进胸腺和脾腺产生抑瘤因子有关。Objective：To investigate the anti-tumor effects of extracts from Orthomorpha on H22 hepatocarcinoma-bearing mice.Methods：H22 hepatocarcinoma-bearing mice model was randomized into 5 groups：model control group,orthomorpha extracts lowdose,medium-dose and high-dose groups（0.40、0.80、1.60g·kg-1）and 5-FU group（5mg·kg-1）.They were given medicine once a day for consecutive 13 days.After the last administration,we investigated the inhibition rate,spleen index,thymus index,AST and ALT activity in serum.Results：In contrast to model group,the inhibition rate of the low,middle,and high dosages of orthomorpha extracts were 27.14%（P〈0.05）,35.31%（P〈0.01）and 40.25%（P〈0.01）,respectively.The spleen index and thymus index of high,middle and low dosages of orthomorpha extracts increased remarkably（P〈0.05）.Conclusion：Orthomorpha extracts could obviously inhibit tumor growth of H22 in tumor-bearing mice and protect their immune function,which maybe involved in the promotion of the thymus and spleen glands tumor suppression factor.

关 键 词：山蛩 肝癌H22 抗肿瘤 免疫 

分 类 号：R285.5[医药卫生—中药学]