microRNA-483和microRNA-486在克隆和转fat-1基因牛组织中的表达  

Expression of microRNA-483 and microRNA-486 in the Cloned and fat-1-transgenic Bovine

作　　者：吕洋[1] 王煜[1,2] 孙佳佳[1] 弓春玲 李光鹏[1] 

机构地区：[1]内蒙古大学实验动物研究中心,呼和浩特010070 [2]内蒙古医科大学附属医院,呼和浩特010050

出　　处：《生物技术通报》2016年第3期105-108,共4页Biotechnology Bulletin

基　　金：国家基础研究计划(2012CB722306);国家级大学生创新训练计划项目(201410126034)

摘　　要：利用荧光定量PCR比较正常受精牛、克隆牛和转基因牛的心、肝、脾、肺、肾、胎盘、子叶、子宫内膜中miR-483和miR-486的表达水平。结果显示,miR-483和miR-486在正常受精牛、克隆牛和转fat-1基因牛的组织中均有表达,其中在心脏中表达量显著高于其他组织。而转fat-1基因牛心脏中miR-483和miR-486表达量均低于正常牛。miR-483和miR-486在不同组织中表达量存在一定差异,在心脏中呈现高表达,提示miR-483和miR-486表达降低可能与心肌肥大、心肌梗死等病理生理过程有关。The expression levels of microRNA-483 and microRNA-486 in heart,liver,spleen,lung,kidney,placenta,cotyledons,endometrium from normal,cloned,and transgenic bovine were measured by real time quantitative PCR. The results showed that microRNA-483 and microRNA-486 were expressed in all tissues of normal,cloned and fat-1-transgenic bovine;significantly higher in the heart than other tissues. However,the expression levels of microRNA-483 and microRNA-486 in transgenic bovine were lower than normal one. Further,the expressions of microRNA-483 and microRNA-486 varied in different tissues and high in the heart,indicating that the expressions of microRNA-483 and microRNA-486 may be correlated with pathophysiological process of myocardial hypertrophy and myocardial infarction.

关 键 词：miR-483 miR-486 转基因牛 心脏 

分 类 号：S823[农业科学—畜牧学] Q78[农业科学—畜牧兽医]