七鳃鳗水通道蛋白3的克隆与生物学特性分析  

Cloning and Biological Characterization of Aquaporin 3 from Lampetra japonica

作　　者：赵春晖[1,2] 史金杰 王浩[2] 刘欣[2] 李庆伟[2] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁省生物技术与分子药物研发重点实验室,大连116081 [2]辽宁师范大学七鳃鳗研究中心,大连116081

出　　处：《生物技术通报》2016年第3期129-136,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31170353)

摘　　要：水通道蛋白3(aquaporin 3,AQP3)属于水甘油通道蛋白家族,参与水、尿素和甘油的转运和促进细胞增殖及迁移。从七鳃鳗中克隆得到了Aqp3(LjAqp3)cDNA(Gen Bank序列号KR054618),其开放阅读框为900 bp,编码299个氨基酸,含6个跨膜螺旋结构域,有信号肽。序列分析结果表明,七鳃鳗AQP3有2个高度保守的天冬酰胺-脯氨酸-丙氨酸(NPA)模体和1个芳香族/精氨酸(ar/R)结构。进行同源序列比对发现与其它物种具有较高的同源性。实时荧光定量PCR分析表明,Lj Aqp3在七鳃鳗中分布广泛,在多种组织器官中表达。Aquaporin 3（AQP3）belongs to the family of aquaglyceroporin,and it involves in the transport of water,urea and glycerol and promotes cell proliferation and migration. In this study,the complete c DNA sequence of Aqp3（designated as LjAqp3）was cloned from Arctic lamprey Lampetra japonica（Gen Bank accession number KR054618）. The open reading frame of LjAqp3 was 900 bp,encoding 299 amino acids,with a predicted 6 transmembrane helix domains and a putative signal peptide. There were 2 highly conserved asparagine-prolinealanine（NPA）motifs and the aromatic/arginine（ar/R）structure in LjAQP3. Additionally,LjAQP3 shared high sequence homology with AQP3 of other species. Real-time quantitative PCR revealed the constitutive expression of LjAqp3 in multiple tissues and organs.

关 键 词：水通道蛋白3 七鳃鳗 天冬酰胺-脯氨酸-丙氨酸模体 

分 类 号：Q78[生物学—分子生物学] Q51