采用蚕豆根尖细胞微核技术检测核设施周围水域的遗传毒性  

Use of the Vicia foba root tip micronucleus test to monitor genotoxicity of river samples from around nuclear facilities

作　　者：邓冰[1] 穆龙[1] 周银行[1] 

机构地区：[1]中国工程物理研究院核物理与化学研究所,四川绵阳621900

出　　处：《癌变．畸变．突变》2016年第2期141-144,148,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：核物理与化学研究所创新基金(2013CX02);中国工程物理研究院科学技术发展基金(2014B0301035)

摘　　要：目的:利用蚕豆根尖细胞微核技术对核设施周围水域的水质污染情况进行检测。方法:分别于芙蓉溪桥河水、涪江河水、安昌河水等7个位点进行水样采集,以采集水样直接处理蚕豆根尖,测定各采样点水样的蚕豆根尖微核率(MNF)及污染指数(PI)和放射性水平,并进行了统计分析。结果:各采样点放射性污染水平均低于国家标准限值,但各采样点的MNF有显著差异,7个采样点中有5个采样点的PI在2以上,和对照组相比差异有统计学意义(P<0.01)。结论:核设施周围水域污染主要是由两岸工业、生活和垃圾等排污引起,核设施运行所产生的放射性流出物对公众和环境的影响轻微。OBJECTIVE： The micronucleus test with Vicia faba root tip cells was used to study genotoxicity of pollutants from rivers around nuclear facilities. METHODS：7 samples collected from river of Furong bridge,Fujiang,Anchang,etc. were used to treat V. faba root tip cells directly. Micronuclei frequencies（MNF） and proliferation index（PI） were determined and statistics analyses were performed to evaluate the difference in MNF among different samples. RESULTS：There was significant differences in MNF for every site. PI values of 5 sites among 7 sampled sites were over 2,and it is highly significant compared with the contrast one（P〈0.01）. CONCLUSION：The results show that the sampling points of radiation pollution levels were lower than the national standard limit value. However,water pollution of the rivers around the nuclear facilities was mainly caused by cross-strait industrial, life,and gabage pollution. Radioactive effluent generated by nuclear facilities operation affected the public and the environment lightly.

关 键 词：蚕豆根尖细胞 微核率 水体污染指数 核设施 

分 类 号：Q345[生物学—遗传学]