纳米银介质中头孢泊肟酯的化学发光特性及分析应用  

Chemiluminescence Characteristics and Analytical Application of Cefpodoxime in Silver Nanoparticles Media

作　　者：张向亮[1] 马红燕[1] 王奕璇[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《延安大学学报（自然科学版）》2016年第1期30-33,共4页Journal of Yan'an University：Natural Science Edition

基　　金：陕西省科技厅自然科学基础研究计划项目(2014JM2056)

摘　　要：在碱性环境中,纳米银能增敏鲁米诺-铁氰化钾体系化学发光信号,然而头孢泊肟酯的加入能使该反应发光强度显著降低。据此,提出了检测头孢泊肟酯的化学发光分析新方法,对其发光机理进行了初步研究。在选取的流路和实验条件下,该方法测定头孢泊肟酯的线性范围为8.0×10^(-9)~1.0×10^(-6)mol/L,方法检出限为4.5×10^(-9)mol/L。对1.0×10^(-6)mol/L和1.0×10^(-7)mol/L的头孢泊肟酯标准溶液平行测定11次,其RSD分别为1.6%和1.2%。方法应用于片剂中头孢泊肟酯的测定,结果满意。It is found that the silver nanoparticles（ Ag NPs）,synthesized by chemical reduction method,could enhance the chemiluminescence intensity of the luminol- K3[Fe（ CN）6]system and the addition of cefpodoxime inhibited the chemiluminescence signals in alkaline solution obviously. Based on these findings,a simple flow- injection chemiluminescence method was reported for the determination of cefpodoxime firstly. The experimental conditions that influence the chemiluminescence intensity were carefully studied and the possible chemiluminescence mechanism had been proposed according to the UV- visible absorption and chemiluminescence spectra. Under the optimum conditions,the linear range for the determination of cefpodoxime was from 8. 0 × 10^-9 mol / L to 1. 0 × 10^-6 mol / L with a detection limit（ 3σ） of 4. 5 × 10^-9 mol / L. The relative standard deviations（ RSD） for the determination of 1. 0 × 10^-6 mol / L and 1. 0 × 10^-7 mol / L cefpodoxime were 1. 6% and 1. 2%,respectively. The method has been used for the determination of cefpodoxime in samples with satisfactory results.

关 键 词：纳米银 头孢泊肟酯 鲁米诺 流动注射 化学发光 

分 类 号：O657.3[理学—分析化学]