拟南芥CWINV1启动子GUS表达载体构建及转基因植株的鉴定  

The GUS Expression Vector Construction of Arabidopsis CWINV1 Promoter and Identification of Transgenic Plants

作　　者：程姣[1] 黄弈[1] 任春梅[1,2] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]作物基因工程湖南省重点实验室,长沙410128

出　　处：《作物研究》2016年第3期237-240,共4页Crop Research

基　　金：国家自然科学基金项目(30671121);国家级大学生研究性学习和创新性实验计划项目((G)SCX1512)

摘　　要：CWINV是编码细胞壁蔗糖转化酶的基因。为深入探讨CWINV基因的表达调控机理,构建了拟南芥CWINV1基因启动子的GUS融合表达载体并转入拟南芥,获得了转基因植株。GUS染色的结果进一步验证了重组表达载体的正确性和实用性。CWINV gene encoding the cell wall invertase. CWINV play an important role in plant growth and development,especially blooming,fruit setting,seed formation and other reproductive development period. But its function has not been elucidated in detail. In order to further exploring the mechanism of CWINV gene expression and regulation,the plant expression vectors of CWINV1 gene promoter was constructed with GUS fused report genes,and then transformed into Arabidopsis,transgenic plants were obtained. GUS staining results further verifies the correctness and practicability of the recombinant expression vector.

关 键 词：拟南芥 CWINV1 载体构建 GUS染色 

分 类 号：Q78[生物学—分子生物学]