模拟失重后高＋Gx暴露对猴牙龈组织热休克蛋白70表达的影响  

Effects of ＋ Gx exposure after simulated weightlessness on HSP70 expression in gingiva of monkey

作　　者：史亮 施生根 王亚宇 洪彪 张建中 包博 张凯 尚乐 

机构地区：[1]解放军第三○六医院全军口腔疾病诊治中心,北京100101 [2]解放军第三○六医院病理实验中心,北京100101

出　　处：《中华航空航天医学杂志》2015年第4期268-271,F0003,F0004,共6页Chinese Journal of Aerospace Medicine

基　　金：国家自然科学基金（11202229）

摘　　要：目的 探讨模拟失重30 d后高＋Gx暴露对猴牙龈组织结构和热休克蛋白70 （heatshock protein 70,HSP70）表达的影响. 方法 11只雄性猕猴按随机数字表法分为4组,即正常对照组（A组,2只）、30 d模拟失重组（B组,3只）,＋13 Gx暴露组（C组,3只）、模拟失重30 d后＋Gx暴露组（D组,3只）.其中D组根据＋Gx峰值又分为＋11 Gx/270 s（D1）、＋13 Gx/230 s（D2）和＋15 Gx/200 s（D3）3组,每组1只.动物放血处死,组织取材后,经4％甲醛固定,石蜡包埋切片,采用组织病理学和免疫组织化学的方法,观察猴牙龈组织结构及HSP70的表达情况. 结果 A组可见正常的牙龈组织结构,其它组牙龈组织结构基本正常,无明显改变.A组牙龈组织HSP70呈阴性表达,上皮和结缔组织均无明显着色;B、C组呈弱阳性表达,牙龈上皮着色弱阳性,结缔组织无明显着色;D组牙龈组织呈阳性表达,牙龈上皮着色阳性,结缔组织无明显着色.统计学显示D组比B组和C组阳性表达量明显增高（P＜0.01）,D3组比D1和D2组阳性表达量明显增高（P＜0.01）. 结论 30 d模拟失重和高＋Gx暴露都诱导猴牙龈组织产生HSP70,模拟失重后高＋Gx暴露显著增加了HSP70的表达.Objective To study possible influence of high ＋-Gx exposure on HSP70 expression in gingiva of the rhesus monkey after 30-day simulated weightlessness.Methods Eleven male rhesus monkeys were randomly divided into four groups：control groups （A,n=2）,30 d simulated weightlessness group （B,n=3）,＋13 Gx group （C,n=3） and high ＋Gx exposure after 30 d simulated weightlessness group （D,n=3）.The group D was divided into 3 subgroups according to Gx peak loads：＋11 Gx/270 s （D1）,＋13 Gx/230 s （D2） and ＋15 Gx/200 s （D3）.The tissues of sacrificed animals were sampled and were fixed with 10% buffered formaldehyde and embedded in paraffin.The paraffin-embedded sections were analyzed by histopathological and immunohistochemical methods to observe the gingival tissue structure and the expression of HSP70.Results Group A showed normal structure of the gingival tissue and other groups＇ were also normal and without obvious change.Group A showed negative expression of HSP70 that the gingival epithelium and connective tissue had no significant stain detected;Group B and C expressed weak positive expression that the stain of gingival epithelium was weak positive and the connective tissue had no significant stain.The gingival tissues of group D showed positive expression that the stain of gingival epithelium was positive and the connective tissue had no significant stain.The expression quantity of HSP70 in the D group was significantly higher than that in group B and C （P〈0.01）.Group D3 had significantly higher positive expression quantity than group D1 and D2 （P〈0.01）.Conclusions Thirty-day simulated weightlessness and high ＋Gx exposure would induce the significance of HSP-70 expression in gingival tissues of rhesus monkey.

关 键 词：失重模拟 加速度 猕猴属 牙龈 组织 HSP70热休克蛋白质类 

分 类 号：R856.78[医药卫生—航空、航天与航海医学]