模拟失重后高＋Gx暴露对猴腮腺组织唾液淀粉酶表达的影响  

Effects of the high ＋ Gx exposure after simulated weightlessness on the expression of salivary alpha-amylase in parotid gland of monkey

作　　者：郭杨 牛忠英 汤楚华 张建中[2] 郑燕华[3] 

机构地区：[1]解放军第三○六医院全军口腔疾病诊治中心,北京100101 [2]解放军第三○六医院病理科,北京100101 [3]解放军第三○六医院特种医学实验中心,北京100101

出　　处：《中华航空航天医学杂志》2015年第4期272-277,共6页Chinese Journal of Aerospace Medicine

基　　金：国家自然科学基金（30870598）;北京市科学技术委员会基金（z131100004013050）

摘　　要：目的 探讨模拟失重30 d后高＋Gx暴露对猕猴腮腺组织中唾液淀粉酶（salivary alpha-amylase,sAA）表达的影响,为航天员口腔健康维护和保健提供试验依据. 方法 选用雄性猕猴23只,依据文献综述及航天飞行实际,按随机数据表法分为4组：①正常对照组（3只）;②模拟失重30 d组（3只）;③＋13 Gx/230 s暴露组（3只）;④模拟失重30 d后高＋Gx暴露组（D组）,14只,依据＋Gx峰值又分为4个亚组,分别是＋11GJ270 s暴露组（D1组,3只）,＋13 Gx/230 s暴露组（D2组,4只）,＋15 Gx/200 s暴露组（D3组,4只）,＋13 Gx/230 s暴露后恢复9d组（D4组,3只）.建立失重模型时,使猕猴头-10°低位卧床.建立高＋Gx暴露模型时,将动物固定于58型动物离心机上,在计算机控制下模拟抛物线型过载曲线,进行相应峰值的＋Gx暴露.各组实验暴露后1d取猕猴腮腺组织,通过Western blot和实时定量聚合酶链反应技术分别检测sAA在蛋白水平及mRNA水平的表达情况.采用SPSS 17.0软件对数据进行统计分析. 结果 在蛋白水平,与对照组相比,各实验组猕猴腮腺组织sAA表达差异有统计学意义（F=80.381,P＜0.01）.模拟失重后再高＋Gx暴露,猕猴腮腺组织sAA表达呈现随着＋Gx值增大而减弱的趋势,而恢复9d后表达回升.sAA在mRNA水平上与其蛋白水平变化一致. 结论 模拟失重30 d后高＋Gx暴露,可造成猕猴腮腺组织中sAA表达减少,唾液成分和功能发生改变.Objective To explore the effects of the high ＋ Gx exposure after 30-d simulated weightlessness on salivary alpha-amylase （sAA） expression in rhesus monkey parotid,in order to provide experimental reference for maintaining astronauts＇ oral health and health care.Methods According to the literature review and the actual situation of spaceflight,twenty-three healthy male rhesus monkeys were randomly divided into four groups：①control group （n=3）;②30-d simulated weightlessness group （n=3）;③＋ 13 Gx/230 s group （n=3）;④group D：high ＋Gx after 30 d simulated weightlessness exposure group （n =14）.The group D was further divided into four subgroups according to the ＋Gx peak load：-＋-11 Gx/270 s （group D1,n=3）,＋13 Gx/230 s （group D2,n=4）,＋15 Gx/200 s （group D3,n=4）,＋-13 Gx/230 s and 9 d＇s recovery （group D4,n=3）.For building weightlessness model,the rhesus monkeys were put on bed with-10° head down tilt position to simulate the microgravity environment.The rhesus monkeys were fixed in Model-58 animal centrifuge exposing to high ＋Gx environment that was built by computer controlled para-hypergravity profile.Salivary glands were collected at the second day of experimental operation.The expression of a-amylase on protein and mRNA level was separately detected by Western blot and quantitative realtime polymerase chain reaction （qRT-PCR）.All of the statistical analysis data were performed by using SPSS 17.0.Results On protein level,Western blot analysis showed that sAA expression in other experimental groups decreased significantly as compared with that in control group （F=80.381,P〈0.01）.In groups of high ＋Gx exposure after simulated weightlessness,sAA expression showed a decreasing trend with the ＋Gx increase.However it rose again by 9-d recovery.On mRNA level,it displayed a conformably trend with that of protein level.Conclusions High ＋Gx exposure after 30-d simulated weightlessness can down-regulate sAA expression in parotid gland of rhesus

关 键 词：失重模拟 超重力 猕猴属 腮腺 唾液α淀粉酶 

分 类 号：R854[医药卫生—航空、航天与航海医学]