甘草废渣中总黄酮提取工艺研究  

Optimization of Extraction Parameters of Total Flavonoids from Licorice Residue

作　　者：刘铭[1] 贾东升[1] 赵江丽 温春秀[1] 崔施展 李荣乔 谢晓亮[1] 

机构地区：[1]河北省农林科学院经济作物研究所,药用植物研究中心,河北石家庄050051 [2]河北省药品检验研究院,河北石家庄050011

出　　处：《食品研究与开发》2015年第23期73-76,共4页Food Research and Development

基　　金：河北省农林科学院青年基金项目(A2015050101);河北太行山区特色功能保健食品的开发研究(11231005D);河北省中药材产业体系项目(11232025D)

摘　　要：优化甘草废渣中总黄酮的提取工艺。以甘草苷为对照品采用比色法测定总黄酮含量,在单因素基础上采用Box-Benhnken响应面法以总黄酮提取得率(Y,%)为响应值,以乙醇浓度(X_1,%)提取时间(X_2,min)、液料比(X_3,mL/g)及提取次数(X_4,次)为自变量,研究各自变量及各自变量间交互作用对总黄酮提取得率的影响,对3批甘草废渣进行验证试验。研究结果表明甘草黄酮最佳提取工艺为:乙醇浓度为70%、提取时间120 min、液料比10:1(mL/g)、提取次数2次,在优化条件下对3批甘草废渣进行工艺验证,甘草黄酮提取得率为(2.4±0.34)%(n=3)与预测理论值2.39%接近。利用Box-Behnken响应面法优化得到的甘草废渣总黄酮提取工艺参数可靠,方法简便,预测性良好。Extraction parameters of total flavonoids from licorice residue was optimized by response surface method. The total flavonoids content was determined by colorimetric method. The effect ofethanol concentration,extraction time, the ratio of material to liquid ratio and extraction times was studied. The effect of each factor and interaction on the extraction rate of total flavonoids was studied. 3 batches of licorice residue were used for validation test under the optimized conditions. Research results showed that thebest extraction parameters was：ethanol concentration 70 %, extracting time 120 min, ratio of 10 ∶ 1（m L/g）, extracting times of 2, the extraction rate of flavonoids was（2.4±0.34） %（n=3）, the difference was little between 2.39 % predictions.The extraction parameters of total flavonoids from licorice residue obtained by Box-Behnken response surface method were reliable, simple and convenient.

关 键 词：甘草废渣 响应面 总黄酮 

分 类 号：TQ28[化学工程—有机化工]