角鲨烯的高效液相色谱检测法  

Determination of Squalene by High Performance Liquid Chromatography with Photodiode Array Detector

作　　者：陈伟珠[1,2] 晋文慧[1] 张怡评[1] 洪专[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]厦门大学化学化工学院化学系化学生物学福建省重点实验室,福建厦门361005

出　　处：《食品研究与开发》2015年第24期131-133,181,共4页Food Research and Development

基　　金：福建省科技计划项目(2015N0009);厦门海洋研究开发院共建项目(2014);海洋生物技术产业化中试技术研发公共服务平台(12PZP001SF10);广东海洋经济发展区域示范项目(GD2012-D01-001)

摘　　要：建立了一种高效液相色谱法(HPLC)测定角鲨烯含量的检测方法。采用XBridge RP C18色谱柱(250 mm×4.6 mm,3.5μm),流动相为甲醇,流速为1.0 m L/min,检测器为光电二极管阵列检测器,检测波长为210 nm。结果表明,在上述色谱条件下,角鲨烯浓度在2.6μg/m L^260μg/m L范围内线性关系良好,相关系数r为0.999 9。检出限(S/N=3)为0.33μg/m L,定量限(S/N=10)为0.99μg/m L,精密度RSD(n=6)为0.40%;平均加标回收率为100.78%,相对标准偏差为0.75%。方法准确、灵敏、可靠,可用于角鲨烯的定量和定性分析。A high-performance liquid chromatography method for the determination of squalene was developed.The determination of squalene was performed on a XBridge RP C18column（250 mm× 4.6 mm,3.5 μm）. The mobile phase was methanol,and the flow rate was 1.0 m L/min.The detector was photodiode array detector and the variable wavelength detector（VWD） was set at 210 nm.In the above chromatographic conditions,good linear was observed in the linear range of 2.6 μg/m L-260 μg/m L, and the correlation coefficient r was 0.999 9.The limit of detection（LOD） of squalene was 0.33 μg/m L（S/N=3）.The quantitation of detection（QOD） of squalene was 0.99 μg/m L（S/N=10）.The relative standard deviation（RSD） for the accuracy was 0.40 %（n=6）and the average recovery was 100.78 % with RSD of 0.75 %.The method is sensitive,reliable,accurate and could be used for the determination of squalene.

关 键 词：角鲨烯 高效液相色谱 光电二极管阵列法 测定 

分 类 号：O657.72[理学—分析化学]