辽宁省2008～2014年流行腮腺炎病毒SH和HN基因特征分析  

SH and HN Protein Genetic Characterization Analysis of Mumps Virus Isolated in Liaoning Province from 2008 to 2014

作　　者：王艳[1] 马艳[1] 郝爽[1] 徐晓婷[1] 韩悦[1] 姚文清[1] 赵卓[1] 

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005

出　　处：《病毒学报》2016年第2期179-184,共6页Chinese Journal of Virology

基　　金：国家科技重大专项"传染病监测技术平台"项目(项目编号:2012ZX10004209)

摘　　要：为了解辽宁省流行的腮腺炎病毒基因特征,本研究将辽宁省2008~2014年分离到32株流行性腮腺炎病毒（Mumps virus,MuV）,应用逆转录-聚合酶链反应（Reverse Transcription Polymerase Chain Reaction,RT-PCR）针对SH和HN基因进行扩增,并对产物进行序列测定。将32株MuV与从GenBank下载的世界卫生组织（World Health Organization,WHO）MuV 12个基因型27个参考株一起对SH和HN蛋白基因分子流行病学研究。除MuVi/Liaoning.CHN/16.11为G基因型外,辽宁省2008~2014年31株MuV分离株均属于F基因型。与疫苗Jeryl-Lynn株和S-79株相比,F基因型MuV在SH基因氨基酸上12个位点发生变异,在HN基因上有27个氨基酸发生变异。F基因型MuV在HN基因464~466位上增加了1个N-糖基化位点,在抗原相关位点121、122、123、279、287、336、356、442上亦发生变异,提示有可能对腮腺炎病毒的抗原性产生影响。To analyze the genetic characterization of epidemic mumps virus strains in Liaoning Province and provide the basis for mumps control.A total of 32 mumps viruses strains were isolated during 2008~2104.The fragment of SH genes and HN genes were amplified by RT-PCR,the PCR products were sequenced and analyzed.Basing on the 316 nucleotides of SH gene,The phylogenetic analyses were processed with the data of WHO mumps reference strains downloaded from GenBank and 32 mumps viruses strains.It showed that the 31 mumps virus strains belong to F genotype except MuVi/Liaoning.CHN/16.11 which was G genotype.Comparing to the A reference strains（Jeryl-Lynn and S-79）,F genotype MuV were mutated on 12 amino acids sites and 27 amino acids siteson on HN gene.F genotype MuV added one N-glycosylation site in 464th-466 th amino acids.The antigenic sites on HN were mutated on 121 th,123th,279 th,287th,336 th,356th and 442 th.Maybe,it will influence the MuV antigenic

关 键 词：腮腺炎病毒 SH蛋白 HN蛋白 基因特征 

分 类 号：R373.16[医药卫生—病原生物学]