弱毒疫苗中鸡传染性贫血病毒低剂量污染对SPF鸡体重和疫苗免疫抗体的影响  

Effect of Low Dose of Chicken Infectious Anemia Virus in Attenuated Vaccine on SPF Chicken Body Weight and Vaccine Immune Antibody

作　　者：房立春[1] 李晓晗[1] 任志浩[1] 李阳[1] 王一新[1] 崔治中[1] 常爽[1] 赵鹏[1] 

机构地区：[1]山东农业大学动物医学院,泰安271018

出　　处：《病毒学报》2016年第2期190-194,共5页Chinese Journal of Virology

摘　　要：为了观察使用污染有低剂量鸡传染性贫血病毒(Chicken infectious anemia virus,CIAV)弱毒疫苗后对鸡免疫和体重的影响,本研究通过人工模拟试验观察了CIAV低剂量污染对SPF鸡体重以及对NDV疫苗抗体产生的影响。结果显示使用每羽份污染10个EID50CIAV和5个EID50CIAV两种剂量的NDV疫苗后均造成了SPF鸡体重的下降,与使用未污染疫苗组相比差异显著;并且使用污染两种剂量CIAV的疫苗后NDV抗体水平与使用无污染组相比也降低了,差异显著。使用污染两种剂量CIAV的NDV疫苗后第2周开始均检测到一定比例的CIAV抗体阳性,而通过核酸检测在使用污染疫苗后第1周就检测到很高比例的CIAV核酸阳性。研究结果不仅展示了弱毒疫苗中CIAV污染对SPF鸡生产性能和免疫机能的影响,也提示我们在通过SPF鸡检查法检测外源病毒污染时增加对病毒核酸检测有助于节省检测时间和提高检出率。In order to observe the effect of the immune and weight of chickens after use the attenuated vaccine with low dose of chicken infectious anemia virus（CIAV）.In this study,the effects of low dose of CIAV on the weight of SPF chickens and NDV antibody production were observed by simulated experiments.The results showed that 10EID50 and 5EID50CIAV per plume attenuated NDV vaccines were used to cause the weight loss of SPF chickens.Compared with the use of the non contaminated vaccine group,it has significant difference.And NDV antibody levels compared with the use of the non contaminated groups also decreased after use the vaccine with two doses of CIAV contaminated.It has significant difference.A certain proportion of CIAV antibody positive was detected at the beginning of the second week after use the NDV vaccine with two doses of CIAV contaminated.The detection of a high proportion of CIAV nucleic acid was detected in the first week after the use of a contaminated vaccine.The results of the study demonstrate the effects of CIAV pollution on the production and immune function of SPF chickens,and it is suggested that increasing the detection of viral nucleic acid can help save time and improve the detection rate in the detection of exogenous virus contamination by SPF chicken test method.

关 键 词：鸡传染性贫血病毒 弱毒疫苗 污染 

分 类 号：S855.3[农业科学—临床兽医学]