番鸭源传染性支气管炎病毒PTFY株的分离鉴定  

The Isolation and Identification of Infectious Bronchitis Virus PTFY Strain in Muscovy Ducks

作　　者：吴晓平[1] 潘书磊[2] 周五朵 吴异健[1] 黄一帆[1] 吴宝成[1] 

机构地区：[1]福建农林大学动物科学学院中西兽医结合与动物保健福建省高校重点实验室,福州350002 [2]福建农业职业技术学院动物科学系,福州350007

出　　处：《病毒学报》2016年第2期203-209,共7页Chinese Journal of Virology

基　　金：农业生物技术专项经费(060856028)

摘　　要：为弄清楚2009年7月某种番鸭养殖场产蛋高峰期番鸭产蛋率、孵化率降低,蛋质量下降的原因,选择无呼吸道症状,死亡率低,剖检可见卵巢肿大充血的番鸭。为确诊该病例,进行病原体的分离与鉴定。取病番鸭卵巢组织,通过鸡胚接种实验、脂溶剂敏感实验、新城疫病毒（Newcastle Disease Virus,NDV）干扰试验、电镜观察和动物接种实验进行病毒分离和鉴定。结果表明：接种鸡胚可导致侏儒胚,该毒株对于脂溶剂敏感,胰酶处理后具血凝性,可干扰NDV复制,电镜下可见典型冠状病毒形态,接种雏鸡可致呼吸道症状,针对N基因部分序列的扩增与分析可见该毒株与传染性支气管炎病毒（Avian infectious bronchitis virus,IBV）参考毒株相应核苷酸序列同源性为84.7%~99%,鉴定所分离毒株PTFY株为IBV,证实IBV对番鸭具有致病性。In July 2009,some farms of breeding Muscovy ducks on the peak of egg laying suffered the decrease of hatching rate and the quality of the eggs showing low mortality and no evident respiratory symptoms.The swelling and congestive ovary was visible after autopsy.This study was brought out for the diagnosis of these cases.The virus was isolated and identified by the methods of virus culture in chicken embryo,physical and chemical properties test,hemagglutinin test,NDV（Newcastle diseases Virus）interference test,electron microscope observation,pathogenicity test and the gene sequence analysis.The results indicated the virus showed the characters of inducing dwarf embryo after inocubation,the sensibility to lipid solvent and the hemagglutination capacity after pancreatic enzyme treatment,the typical morphology of coronavirus,the interference to NDV replication and the homology among 84.7% ~ 99% of the particial N gene sequences to the reference IBV（Avian infectious bronchitis virus）strains.The strain was identified as IBV isolate and this study confirmed the pathogenicity of IBV to Muscovy ducks.

关 键 词：传染性支气管炎病毒 番鸭 分离鉴定 

分 类 号：S852.65[农业科学—基础兽医学]