3种园林植物土壤真菌群落结构及影响因子  

Response of soil fungi community to different garden plants

作　　者：李金前[1,2] 赵静杰[1] 王吉[1] 刘亚军[1,2] 钟丽梅[1,2] 吴兰[1,2] 

机构地区：[1]南昌大学生命科学学院,江西南昌330031 [2]江西省分子生物学与基因工程重点实验室,江西南昌330031

出　　处：《南昌大学学报（理科版）》2015年第5期492-497,共6页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(31360127)

摘　　要：采用传统的培养法与变性梯度凝胶电泳分子技术相结合,对3种园林植物(海桐、雪松、桂花)土壤真菌群落结构进行了研究;并分析了土壤性质与真菌数量和群落结构关系。结果表明:3种园林植物土壤中的真菌数量和群落结构差异显著。其中,海桐样地土壤可培养真菌数量最高,雪松次之,桂花样地最低;聚类分析表明,相比于桂花,海桐和雪松土壤真菌群落结构具有更高的相似度。不同园林植物土壤理化性质显著不同。与桂花样地相比,海桐和雪松样地的土壤性质相更为相近。相关性和冗余分析结果显示:可培养的真菌数量与土壤含水量显著正相关;群落结构显著受土壤含水量、pH、总有机碳和总氮影响。结果提示,土壤含水量是导致不同园林植被土壤真菌群落结构不同的关键环境因子。The soil fungi communities under three garden plants（Pittosporum tobira,Cedrusdeodara,Osmanthusfragrans）were investigated by using colony counting and denaturing gradient gel electrophoresis（DGGE）method.The effects of edaphic factors on soil fungal abundance and structure were evaluated.It was found that the numbers of fungi and community structure were significantly different among soils from the three plant species.The soil fungi abundance of P.tobira was the highest,thenC.deodara and O.fragrans.Soil fungal communitycluster analyses revealed that the community structures of P.tobira and C.deodara had higher similarity,but different from that of O.fragrans.2.Soils from different plant species significantly varied in soil moisture,pH,total organic carbon and total nitrogen content.The soil property of P.tobira was similar to that of C.deodara,but unlike O.fragrans.3.Correlation analysis showed thatthe abundance of culturable soil fungi significantly correlated with soil moisture content.Furthermore,the redundancy analysis（RDA）suggested that soil moisture content,pH,total organic carbon and total nitrogen had significant effects on soil fungal community structure;particularly,soil moisture was the key factor for differences of the soil fungi community structure of the three garden plants.

关 键 词：园林植物 土壤真菌 变性梯度凝胶泳 影响因子 

分 类 号：Q939.96[生物学—微生物学]