不同大孔树脂对粉被虫草中N^6-(2-羟乙基)腺苷的分离特性研究  

Desorption Characteristic of N^6-(2-hydroxyethyl )-Adenosine from Cordyceps pruinosa Petch by Different Macroporous Resin

作　　者：唐远江 雷帮星[2] 康冀川[2] 刘玉秋[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025

出　　处：《山地农业生物学报》2015年第6期53-57,78,共6页Journal of Mountain Agriculture and Biology

基　　金：贵州省社发攻关项目"三种虫草资源镇痛组分研究"[黔科合SY字(2008)3047];贵州省科技计划项目"生物活性成分智能化中试提取平台建设及在产品开发中的应用"[No.黔科合高G字(2010)4009]

摘　　要：为选择合适的大孔树脂分离粉被虫草中的N6-(2-羟乙基)腺苷(HEA),通过静态吸附与解吸附试验对4种大孔树脂吸附和解吸附HEA的效果进行比较,并对洗脱条件进行优化。结果表明DM-130树脂对HEA的吸附量最大、解吸率较高;该树脂的最佳动态吸附条件是上样量为2倍树脂床体积(BV),吸附时间为6 h;最佳动态洗脱条件是依次用3 BV蒸馏水,1 BV 5%乙醇和3.5 BV 10%乙醇冲洗柱床。在该条件下,经HPLC分析测定,洗脱液中HEA的峰面积百分比达到89.15%。In order to select the appropriate macroporous resin to isolate N^6-（2- hydroxyethyl）- Adenosine from Cordyceps pruinosa Petch,tests using four kinds of macroporous resin to absorb and desorb HEA were adopted,and the elution conditions were also optimized. The results showed that DM- 130 resin had the highest resolution and greater adsorption for HEA. The best elution conditions for dynamic adsorption were as follows： the loading amount was 2 times bed volume（ BV） and the adsorption time was 6 h; the best elution conditions were that DM- 130 resin should be eluted sequentially with 3 BV water,1 BV 5% ethanol and3. 5 BV 10% ethanol. HPLC analysis showed that under the optimum conditions peak area percentage of HEA was 89. 15% in eluent.

关 键 词：大孔树脂 N6-(2-羟乙基)腺苷 吸附 解吸附 

分 类 号：R931[医药卫生—生药学]