辛伐他汀干预卵巢切除大鼠骨密度和生物力学性能的变化  

Effect of simvastatin on bone mineral density and biomechanical properties of ovariectomized rats

作　　者：张岩 刘昊 邢磊 张国彬 田发明 

机构地区：[1]华北理工大学附属医院,河北省唐山市063000 [2]华北理工大学研究生学院,河北省唐山市063000 [3]华北理工大学医学实验研究中心,河北省唐山市063000

出　　处：《中国组织工程研究》2016年第7期981-986,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省高等学校科学研究计划(QN20131007);河北省自然科学基金(H2013209255);唐山市老年医学重点实验室资助项目(14140221B)~~

摘　　要：背景:降脂类药物辛伐他汀表现出成骨作用潜能,有望成为新型促成骨类药物,但其体内干预作用效果尚存争议,观察部位的选择是否影响实验结论尚无确切证据。目的:观察辛伐他汀体内给药对卵巢切除大鼠骨丢失模型股骨及椎体骨量和生物力学性能的影响。方法:6月龄雌性SD大鼠24只,随机分为3组,每组8只。卵巢切除组、卵巢切除加辛伐他汀干预组大鼠在双侧卵巢切除后第2天分别接受安慰剂和辛伐他汀5 mg/(kg/d)干预;假手术组只暴露卵巢,不予切除,直接缝合。8周后处死所有大鼠,采用ELISA法检测血清Ⅰ型前胶原氨基端前肽水平,取第4,5腰椎和股骨,双能X射线法检测大鼠第5腰椎和左侧股骨骨密度,压缩试验(椎骨)和三点弯曲试验(股骨)检测第4腰椎和右侧股骨最大载荷、弹性模量等生物力学指标。结果与结论:(1)卵巢切除组和卵巢切除+辛伐他汀组Ⅰ型前胶原氨基端前肽水平显著高于假手术组。(2)卵巢切除大鼠第5腰椎和股骨骨密度均显著低于假手术组(P<0.05),卵巢切除+辛伐他汀第5腰椎骨高于卵巢切除组(P<0.05),股骨骨密度高于卵巢切除组,但差异无显著性意义。(3)卵巢切除组大鼠第4腰椎最大载荷、弹性模量均显著低于假手术组和卵巢切除+辛伐他汀组;卵巢切除组股骨弹性模量显著低于假手术组和卵巢切除+辛伐他汀组。结果证实辛伐他汀可部分阻止卵巢切除大鼠骨量丢失,对椎体骨的作用较股骨更显著,椎体较股骨更适于分析生物力学性能。BACKGROUND： Osteoporosis and its complications severely threaten the elder＇s health. Simvastatin, widely accepted as a lipid-lowering drug, is reported to potentially promote bone formation, but it is in debate when orally administered, and there is no evidence to support whether this is due to the region difference. OBJECTIVE： To investigate the effect of orally administered simvastatin on bone mass and biomechanical properties of the femur and vertebrae in osteopenia rats induced by ovariectomy（OVX）. METHODS： Twenty-four 6-month-old female Sprague-Dawley rats were subjected to OVX＋orally administered saline vehicle（OVX group, n=8）, OVX＋orally administered simvastatin（5 mg/kg/d; intervention group, n=8） or sham surgery（sham group, n=8）. After 8 weeks of treatment, all rats were sacrificed and the level of procollagen type I N-terminal propeptide in blood serum was assessed by ELISA. Bone mineral density was determined in the L5 vertebra and left femur using dual-energy X-rays. Furthermore, the biomechanical properties of the L4 vertebra and right femur, including maximum load and elastic modulus, were detected by compression testing and three-point bending test, respectively. RESULTS AND CONCLUSION： The serum level of procollagen type I N-terminal propeptide in the sham group was significantly lower than that in the other two groups. OVX rats showed significantly lower bone mineral density in both the L5 vertebra and left femur than sham rats（P 0.05）. Rats in the intervention group showed higher bone mineral density than those in the OVX group, with statistically significant difference in the L5 vertebra（P 0.05）, but insignificant difference in the femur. Maximum load and elastic modulus of the L4 vertebra in the OVX group were significantly lower than those in the sham and intervention group. Markedly lower elastic modulus of the femur was found in the OVX group than the sham and intervention groups. These findings demonstrate that simvastatin treatment can partially preven

关 键 词：骨质疏松 卵巢切除术 骨密度 组织工程 组织构建 骨组织工程 辛伐他汀 生物力学 河北省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]