两相体系中羟化酶催化1,2,3,4-四氢喹啉不对称羟基化反应  

Asymmetric Hydroxylation of 1,2,3,4-Tetrahydroquinoline by Hydroxylase-catalyzed Hydroxylation in Two-phase Reaction System

作　　者：李珂[1] 杨敏[1] 崔宝东 陈永正[1] 

机构地区：[1]遵义医学院药学院,贵州遵义563000

出　　处：《合成化学》2016年第4期350-354,共5页Chinese Journal of Synthetic Chemistry

基　　金：国家自然科学基金资助项目(21162047);贵州省科技厅项目(QKHGZ-2011-7017)

摘　　要：以菌株Pseudomonas plecoglossicidas ZMU-T02整细胞为生物催化剂,1,2,3,4-四氢喹啉经不对称羟基化反应合成了(R)-4-羟基-1,2,3,4-四氢喹啉,其结构经1H NMR,13C NMR,HR-MS和HPLC确证。在细胞浓度为30g cdw·L-1,p H为9.0,整细胞悬浮液/有机溶剂比例为1∶1的两相体系中,底物浓度为10 mmol·L-1时,收率37%,ee值98%。（ R ） -4-hydroxy-1,2,3,4-tetrahydroquinoline was synthesized by hydroxylation of 1,2,3,4- tetrahydroquinoline using the whole-cell of Pseudomonas plecoglossicidas ZMU-T02 as biocatalyst. The structure was confirmed by ^1H NMR, ^13C NMR, HR-MS and HPLC. Under the optimized reaction conditions[ cell density 30 g cdw · L-^1, pH 9.0, 50% （V/V） hexadecane as co-solvent], when substrate concentration was 10 mmol · L^-1 , the yield and ee were 37% and 98% , respectively.

关 键 词：1 2 3 4-四氢喹啉 不对称羟基化 (R)-4-羟基-1 2 3 4-四氢喹啉 生物催化 合成 两相反应 

分 类 号：O626.3[理学—有机化学] O643.3[理学—化学]