洋常春藤法呢基焦磷酸合酶基因的克隆与序列分析  

Cloning and sequence analyzing of farnesyl pyrophosphate synthase from Hedera helix

作　　者：阮琴妹 曹雄军[1,2] 孙化鹏[1] 钟晓红[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]广西农业科学院葡萄与葡萄酒研究所,广西南宁530007

出　　处：《湖南农业大学学报（自然科学版）》2016年第2期136-141,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省研究生科研创新项目(CX2014B290);湖南省教育厅科研项目(15A089)

摘　　要：以洋常春藤叶片为材料,采用同源RT–PCR方法,克隆获得了洋常春藤法呢基焦磷酸合成酶基因,其c DNA序列大小为1 050 bp,开放阅读框为1 026 bp,推测编码342个氨基酸残基,该序列的核苷酸、氨基酸与五加科植物有同源性;用在线数据软件进行分析,推测该蛋白可能存在于细胞质之中,定位在细胞膜外,FPS蛋白的相对分子质量为39 735.7;该蛋白含有2个天冬氨酸保守结构域,其二级结构主要以α螺旋和无规则卷曲为主;双子叶植物系统进化树分析结果表明,洋常春藤的FPS与刺五加、人参、三七、西洋参、辽东楤木的FPS亲缘关系最近,这一结果符合传统的分类法规则。In this study, c DNA encoded farnesyl diphosphate synthase（FPS） was cloned using homology RT–PCR method from Hedera helix leaves. The results revealed that sequence of the c DNA with 1 050 bp base had an open reading frame of 1 026 bp and 342 amino acid residues. The sequence of nucleotides and amino acids of FPS were homology with those of araliaceous plants; according to the online prediction, the FPS, with a 39 735.7 relative molecular mass of the protein, stored in cytoplasm and located in the outer membrane; the speculated FPS contained two conserved domains of aspartic acid, and its secondary structure was mainly dominated with alpha helix and random coil; phylogenetic analysis on amino acid sequence of the FPS in dicotyledons showed that the FPS was closely related to Eleutherococcus senticosus, Panax ginseng, Panax notoginseng, Panax quinquefolius and Aralia elata, which were consistent with the traditional classification rules.

关 键 词：洋常春藤 法呢基焦磷酸合成酶 同源性 五加科植物 

分 类 号：Q943.2[生物学—植物学]