化工厂废水中对甲苯磺酸降解菌的筛选及降解特性研究  

Isolation and Degradation Characteristics of a New p-Toluenesulfonic Acid Degrading Strains from Chemical Plant Wastewater

作　　者：沈洁[1] 李加友[1] 陆筑凤[1] 蔡丽玲[1] 

机构地区：[1]嘉兴学院生化学院,浙江嘉兴314001

出　　处：《浙江化工》2016年第3期34-37,共4页Zhejiang Chemical Industry

基　　金：嘉兴市科技计划项目(2014AY21009);浙江省芳烃磺酸工程技术研究中心开放基金项目(84214001-6)

摘　　要：以对甲苯磺酸为唯一碳源,从化工厂供试废水中筛选出对甲苯磺酸降解菌,经形态学观察和分子生物学鉴定,确定这种菌株为哈茨木霉菌,命名为哈茨木霉1228。利用固态发酵的方法制备哈茨木霉1228,培养物直接投入化工厂供试废水进行处理,结果发现:在p H=5、温度30℃时,处理7 h后,化工厂供试废水中对甲苯磺酸降解率可达45%。The p-toluenesulfonic acid（p-TSA） degrading strain was isoloated from chemical plant wastewater, which can use p-TSA as the sole carbon nutrition for growth.The degrading strain was identified as Trichoderma harzianum based on the results of morphological observation and molecular biological analysis, and it was named Trichoderma harzianum 1228. Trichoderma harzianum 1228 was preparation for treatment of wastewater from chemical plant by solid state fermentation method. The results showed that, the degradation rate of p-TSA can reach 45% when the chemical plant was treated with 5 h at p H=5 and 30 ℃.

关 键 词：对甲苯磺酸 生物降解 哈茨木霉 降解特性 

分 类 号：X78[环境科学与工程—环境工程] X172