芦丁磁性脂质体的制备及质量评价  

Preparation and quality evaluation of magnetic rutin liposomes

作　　者：张辉[1] 胡礼军[1] 喻莉萍[2] 李鑫[1] 何艳[1] 姚飞虹[1] 

机构地区：[1]湘南学院药学院 [2]湘南学院图书馆,湖南郴州423000

出　　处：《湘南学院学报（医学版）》2016年第1期10-13,共4页Journal of Xiangnan University（Medical Sciences）

基　　金：湖南省教育厅科学研究重点项目资助(14A134);湘南学院重点建设学科资助项目(XNV125KD029);湘南稀贵金属化合物及其应用湖南省重点实验室开放基金(2012XGJSYB04)

摘　　要：目的制备芦丁磁性脂质体,并对其包封率、粒径大小和稳定性进行检测。方法采用超声薄膜分散法制备芦丁脂质体;采用化学沉淀法制备Fe3O4颗粒,并利用水合油酸钠包覆沉淀出的磁性颗粒;用超声将芦丁脂质体和磁流体混合分散,制备芦丁磁性脂质体;利用紫外可见分光光度法测定其包封率,并初步考察磁性脂质体的稳定性。结果制备的芦丁磁性脂质体在显微镜下为圆形,直径分布为29.4～39.2μm,包封率为（20.69±4.56）%,粒径大小较为集中,短时间内稳定性较好。结论用超声薄膜分散法制备的芦丁磁性脂质体平均粒径较均匀,外观呈圆形且表面光滑,分布均匀,稳定性较好,但包封率有待提高,制备后4℃保存,渗漏率较低。Objective To prepare magnetic rutin liposomes and detect its encapsulated ratio,particle size and stability. Methods The rutin liposome was prepared by ultrasound film dispersion method. The Fe3O4 magnetic fluid was prepared by the chemical coprecipitation,and coated Fe3O4 particle with acid. Lastly,mix and disperse the rutin liposome and magnetic fluid by means of ultrasonic to prepare magnetic rutin liposomes. There encapsulation efficiency was detected by the ultraviolet- visible absorption spectrometry method,and the stability of magnetic liposome was investigated. Results The magnetic rutin liposomes by orthogonal design were globular,the diameter spread is 29. 4 ～ 39. 2 μm. Its encapsulation efficiency are（ 20. 69± 4. 56） %,and the particle size is more concentrated and the short- time stability is good. Conclusion The magnetic rutin liposomes have well- proportioned diameter by ultrasound film dispersion method,its appearance is globular and surface is smooth,its distribution is uniform and the stability is good. However,the encapsulation efficiency needs to be improved,and when saved at 4℃ after preparation,the leakage ratio is low in the short time.

关 键 词：芦丁 四氧化三铁 脂质体 稳定性 包封率 

分 类 号：R944[医药卫生—药剂学]