三七疫霉病拮抗根际土壤细菌的筛选及初步鉴定  

Screening and Preliminarily Identification of Rhizosphere Soil bacterial Controlling Phytophthora Cactorum of Panax Notoginsing

作　　者：杨丽芬[1] 杨建[1] 高玲玲[1,2] 郭建伟[1,3] 洪亮[1] 程加省[4] 孔琼[1] 田学军[1] 

机构地区：[1]红河学院云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [2]河南省农业科学院农副产品加工研究所,郑州450002 [3]中国科学院新疆生态与地理研究所干旱区生物地理与生物资源重点实验室,乌鲁木齐830011 [4]云南省农业科学院粮食作物研究所/国家小麦改良中心云南分中心,昆明650205

出　　处：《红河学院学报》2016年第2期24-26,36,共4页Journal of Honghe University

基　　金：红河学院硕士点植物保护一级学科建设项目;红河学院中青年学术骨干人才计划项目(2015GG0206)

摘　　要：为了筛选对环境友好的生防细菌防治三七疫霉病,通过高温处理、稀释涂布法分别从三七健康及患病植株根际土壤分离了21和16株可培养细菌,并以对峙培养法分别筛选到6和7株拮抗菌株,上述13株拮抗菌株经生理生化特征鉴定为芽孢杆菌属。结果表明,健康植株根际土壤有较丰富的可培养细菌,而患病植株根际土壤可培养细菌中的拮抗菌株较丰富,表明健康植株与患病植株根际土壤的拮抗细菌丰度发生了变化。解淀粉芽孢杆菌B1的抑菌带宽达8.5mm,具有一定的潜在生防价值。To screen eco-friendly bio-control bacteria controlling Phytophthora cactorum of Panax notoginsing, 21 and 16 strains rhizobacteria were separated respectively from healthy and infected Panax notoginsing by thermal processing, tissue crashing method, dilution coating method, additionally 6 and 7 strains antagonistic bacteria among them were respectively selected by confrontation culture method, then total of antagonistic bacteria above were identified as Bacillium spp. through physiological and biochemical characteristics. The results suggested that there were richer cultural bacteria in rhizosphere soil of healthy plants, in contrast that there were relatively richer antagonistic bacteria in rhizosphere soil of infected plants. In another word, the richness of antagonistic bacteria changed in rhizosphere soil of healthy and infected plants. Bacillus amyloliquefaciens B1 identified by physiological and biochemical characteristics, which had a certain potential bio-control value because of inhibition zone width up to 8.5 mm.

关 键 词：恶疫霉 根际细菌 拮抗作用 

分 类 号：Q939[生物学—微生物学]