HPLC法同时测定承德山里红叶中绿原酸和牡荆素鼠李糖苷的含量  

Determination of Ch Lorogenic Acid and Vitexin Rhamnoside in Major Hawthorn Leaf of Chengde by HPLC

作　　者：杜义龙[1] 潘海峰[1] 

机构地区：[1]承德医学院河北省中药研究与开发重点实验室,河北承德067000

出　　处：《山西职工医学院学报》2016年第1期4-7,共4页Journal of Shanxi Medical College for Continuing Education

摘　　要：目的：建立高效液相色谱（HPLC）法同时测定承德地区山里红叶中绿原酸和牡荆素鼠李糖苷的含量。方法：采用Agi Lent ZORBAX SB-C18色谱柱（4.6 mm×250 mm,5μm）;以乙腈-0.1%甲酸水溶液-四氢呋喃为流动相梯度洗脱;检测波长为340 nm;检测时间50 min;流速1.0 m L·min-1;柱温30℃;进样量10μL。结果：15批承德山里红叶样品中绿原酸和牡荆素鼠李糖苷的线性范围分别为2.56-410、2.51-400 mg·L-1（r分别为0.999 9,0.999 8）;平均加样回收率分别为99.8%（RSD 0.48%）,99.7%（RSD 0.77%）。结论：该方法准确可靠,重现性好,可用于山里红叶的质量控制。Objective： To develop HPLC method for simultaneous determination of chlorogenic acid and vitexin rhamnoside in Major Hawthorn Leaf of Chengde. Method： With an Agilent ZORBAX SB-C18column（ 4. 6 × 250 mm,5 μm）,gradient elution was performed by mobile phase of 0. 1% formic acid-acetonitrile-tetrahydrofuran. The detection wavelength was 340 nm; detection time was 50 min; f Low rate was 1. 0 m L·min- 1; the column temperature was 30℃;injection volume was 10 μL. Results： In the 15 batches of Major Hawthorn Leaf of chengde,the Linear ranges of chlorogenic acid and vitexin rhamnoside were from 2. 56 - 410 mg·L- 1（ r = 0. 999 9） to 2. 51 - 400 mg·L- 1（ r =0. 999 8）,respectively. The average recovery rates of chlorogenic acid and vitexin rhamnoside were 99. 8%（ RSD0. 48%）,99. 7%（ RSD 0. 77%）,respectively. Conclusion： This method is accurate,reproducible and suitable for controlling the quality of Major Hawthorn Leaf.

关 键 词：山里红叶 高效液相色谱法 绿原酸 牡荆素鼠李糖苷 

分 类 号：R917[医药卫生—药物分析学]