聚乙烯亚胺介导Bmk CT基因抑制C6胶质瘤细胞C-Myc、VEGF表达  

Expression of Polyethyleneimine-mediated Bmk CT Gene Inhibitation to VEGF and C-Myc in C6 Glioma Cells

作　　者：张亚涛[1] 范丽娜[1] 温春丽[2] 胡风云[2] 

机构地区：[1]山西中医学院,山西太原030024 [2]山西省人民医院,山西太原030012

出　　处：《山西职工医学院学报》2016年第1期7-10,共4页Journal of Shanxi Medical College for Continuing Education

摘　　要：目的:应用阳离子聚合物聚乙烯亚胺(polyethyleneimine,PEI)介导东亚钳蝎氯离子通道毒素(Buthus martensii Karsch Chlorotoxin-like Toxin,Bmk CT)基因转染C6胶质瘤细胞,观察其对C6细胞内C-Myc、VEGF表达的影响。方法:将PEI分别和p EGFP-N1-Bmk CT、p EGFP-N1质粒混合,获得PEI/p EGFP-N1-Bmk CT和PEI/p EGFP-N1两种复合物,将其转染C6细胞,MTS法观察细胞增殖和存活活力,48 h后通过Western-blot方法检测CMyc、VEGF蛋白表达水平。结果:PEI/p EGFP-N1-Bmk CT转染C6细胞48 h后较PEI/p EGFP-N1能够显著抑制C6细胞增殖和存活活力,同时抑制C-Myc、VEGF蛋白表达。结论:PEI/p EGFP-N1-Bmk CT转染C6细胞可能通过抑制C-Myc、VEGF表达进而抑制C6细胞增殖和血管生成。Objective： To investigate the effects of PEI（ polyethyleneimine）-mediated Bmk CT（ Buthus martensii Karsch Chlorotoxin-like Toxin）,Bmk CT gene transfection on the expression levels of C-Myc and VEGF of C6 glioma cells. Methods： p EGFP-N1 and p EGFP-N1-Bm K CT were combined with PEI to form the composites,PEI / p EGFP-N1-Bmk CT and PEI / p EGFP-N1,which were used to transfect C6 glioma cells. Then,MTS test was performed to investigate the proliferation and viability of the transfected cells. After 48 hours the expression of C-Myc and VEGF proteins were detected by Western-bloting. Results： The proliferation of C6 glioma cells in the p EGFP-N1-Bmk CT group was significantly inhibited,while the expression levels of C-Myc and VEGF were lower than p EGFP-N1 group after the transfection.Conclusion： PEI / p EGFP-N1-Bmk CT may suppress the angiogenesis and the proliferation of C6 glioma cells through inhibiting the expression of C-Myc and VEGF.

关 键 词：BMK CT 胶质瘤 C-MYC VEGF 

分 类 号：Q78[生物学—分子生物学]