半边旗提取物5F诱发结直肠癌细胞生长抑制及细胞凋亡  

Growth inhibition and apoptosis in human colorectal cancer cells induced by 5F-extracted from Pteris semipinnata L.

作　　者：高小胜 吕应年 吴科锋 刘义 符伟玉[3] 陈若泰 李立 

机构地区：[1]广东医科大学第一临床医学院,广东湛江524023 [2]广东医科大学广东天然药物研究与开发重点实验室,广东湛江524023 [3]广东医科大学生物化学与分子生物学教研室,广东湛江524023

出　　处：《药学研究》2016年第4期193-196,211,共5页Journal of Pharmaceutical Research

基　　金：广东省自然科学基金项目(No.S2012010008163);湛江市财政资金科技专项竞争性分配项目(No.2013A01008);广东医学院创新实验项目(No.ZYZF006)

摘　　要：目的检测半边旗(Ps L)提取物5F(ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid)对HCT-116结直肠癌(CRC)细胞的抗增殖及促细胞凋亡效果。方法采用噻唑蓝(MTT)法检测细胞毒性。通过有代表性的细胞凋亡相关蛋白表达及Annexin V/PI染色确定细胞凋亡。采用标准实验方案测量活性氧(ROS)水平。结果 5F以剂量依赖及时间依赖方式抑制HCT-116细胞增殖。免疫印迹分析表明5F提高了HCT-116细胞中的Bax/Bcl-2比值。5F能既够增强顺铂(cisplatin)对HCT-116细胞生长的抑制效果又能够提高顺铂诱导的HCT-116细胞凋亡水平。5F不仅没有提高活性氧水平,反而降低了顺铂诱导的活性氧生成。结论 5F能够诱导结直肠癌细胞凋亡并因此表现出癌症治疗潜力。Objective To examine the antiproliferative and proapoptotic effects of 5F( ent- 11α- hydroxy- 15- oxo- kaur- 16- en- 19- oic- acid),a compound isolated from Pteris semipinnata L.( Ps L),on HCT- 116 colorectal cancer( CRC) cells. Methods Cytotoxicity was examined using a MTT method. Cell apoptosis was determined through expression of representative apoptosis- related proteins and Annexin V/PI staining. Reactive oxygen species( ROS) level was measured using standard protocols. Results 5F inhibited the proliferation of HCT- 116 cells in a concentration- and time- dependent manner. 5F increased the ratio of Bax/Bcl- 2 in HCT- 116 cells was identified by western blotting analysis. 5F could enhance both the inhibitory effect of cisplatin on growth of HCT- 116 cells and the level of apoptosis induced by cisplatin in HCT- 116 cells. 5F did not increase ROS,but reduced ROS production induced by cisplatin. Conclusion 5F can induce CRC cell apoptosis and therefore represents therapeutic potential for cancer treatment.

关 键 词：半边旗 结直肠癌 细胞凋亡 活性氧 

分 类 号：R285[医药卫生—中药学]