饮用水有机提取物对L02细胞增殖及细胞周期的影响  

The Influence on Cell Proliferation and Cell Cycle of L02 Cells Exposed to Organic Extracts from Drinking Water

作　　者：岑延利[1] 杨光红[1] 桂晓玲[1] 张爱华[1] 李传美[1] 

机构地区：[1]贵州医科大学公共卫生学院

出　　处：《贵阳医学院学报》2016年第3期294-297,303,共5页Journal of Guiyang Medical College

基　　金：贵州省科技厅基金[黔科合LH字(2014)7102];贵阳市科学技术计划项目[筑科合同(2013103)19号]

摘　　要：目的:探讨饮用水有机提取物对人正常肝细胞(L02)的细胞增殖及细胞周期的影响。方法:体外培养L02细胞,将其分别暴露于培养液(空白对照组)、0.1%DMSO(溶剂对照组)和0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/m L固相萃取法提取的饮用水有机提取物(染毒组)中24、48及72 h,采用MTT法观察L02细胞的增殖情况,流式细胞仪检测细胞周期的构成比。结果:(1)与溶剂对照组比较,各染毒组细胞活力随着有机提取物浓度的增加及时间的延长而降低,差异有统计学意义(P<0.05);(2)随染毒剂量的增加,细胞周期中G0/G1期细胞比例逐渐下降,除24 h、48 h 0.312 5 L/m L染毒组外,其余剂量染毒组G0/G1期细胞比例均明显低于溶剂对照组(P<0.05);S期细胞比例则随有机提取物浓度的增加而上升,除24、48及72 h 0.312 5 L/m L的染毒组外,其余染毒组中S期细胞比例的增加与对照组比较,差异有统计学意义(P<0.05);染毒48 h、72 h后,L02细胞中G0/G1期细胞比例低于24 h染毒组(P<0.05);S期细胞比例则高于24 h染毒组(P<0.05)。结论:在本研究条件下,饮用水有机提取物可以阻滞L02细胞于S期,抑制细胞的增殖,且呈时间、剂量依赖性。Objective： To investigate the influence of organic extracts from drinking water on liver cell（ L02） proliferation and cell cycle. Methods： The L02 cells was cultivated in vitro： blank control group（ culture solution）,solvent control group（ 0. 1% DMSO）; organic extracts group（ The dose of pollutants groups were 0. 312 5,0. 625 0,1. 250 0,2. 500 0 and 5. 000 0 L / m L samples were extracted by solid phase extraction method）. Each group was treated for 24 h,48 h and 72 h. Cell proliferation was measured by MTT assay. The constituent ratio of cell cycle was detected with flow cytometry.Results：（ 1） Compared with solvent control group,cell vitality of L02 cells decreased gradually with the increasing of exposure time and concentration of organic extracts. The differences were statistically significant（ P〈0. 05）.（ 2） The proportion of cells in G0 / G1 phase decreased gradually with the increasing concentration of organic extracts. Except for 0. 312 5 L / m L of pollutants group at 24 h and48 h,significant differences were found in other exposure groups（ P〈0. 05）. The proportion of cell in S phase increased gradually with the increasing concentration of organic extracts. Except for0. 312 5 L / m L at 24 h,48 h and 72 hr exposure,significant differences existed compared with solvent control group,differences were statistically significant（ P〈0. 05）. Compared with 24 h exposure group,exposed groups at 48 h- exposure and 72 h- exposure demonstrated significantly increase in G0 / G1 phase cell proportion and S phase cell proportion with increasing time（ P〈0. 05）. Conclusions： The results of the present paper showed that the organic extracts from drinking water can inhibit cell proliferation and change cell cycle of L02 cells.

关 键 词：饮用水 有机提取物 肝细胞 细胞增殖 细胞周期 

分 类 号：R114[医药卫生—卫生毒理学]