无创血液成分检测中基于VIP分析的波长筛选  

Study on the Wavelength Selection Based on VIP Analysis in Noninvasive Measurement of Blood Components

作　　者：贺文钦 严文娟[3] 贺国权[3] 杨增宝[3] 谭勇[3] 李刚[1,2] 林凌[1,2] 

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室,天津300072 [2]天津大学天津市生物医学检测技术与仪器重点实验室,天津300072 [3]长江师范学院电子信息工程学院,重庆408100

出　　处：《光谱学与光谱分析》2016年第4期1080-1084,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30973964);重庆市自然科学基金项目(CSTC2011JJA10113);天津市应用基础及前沿技术研究计划项目(11JCZDJC17100)资助

摘　　要：自变量筛选是定量光谱分析领域的研究热点,简便且高效的自变量筛选方法不但可以降低分析计算量,提高分析精度,同时还可以减轻对仪器光谱分辨能力的依赖,降低分析成本。波长筛选也是光谱法无创血液成分检测研究的重要环节。动态光谱理论为血液无创检测提供了极佳的思路,但长期局限于使用宽带光源和高分辨率的光谱仪器,分析中需要大量波长限制了动态光谱法的进一步发展。为了去除冗余信息,使检测走向低成本化和集成化,提出了基于变量投影重要性(variable importance in projection,VIP)分析的波长筛选方法。通过分析PLS模型中各维自变量对因变量的解释能力,从而剔除重要性较低的变量保留解释能力强的波长。以232例受试者的临床实验数据为基础,以血红蛋白含量为分析对象,经投影重要性分析后将波长数由586降至64,波长筛选后血红蛋白预测模型的测试集平均相对误差(MREP)为1.82%,使用了极少的波长便可得到满意的结果;结合Bootstrap方法对模型进行显著性检验后验证了波长变量的解释能力。首次指出了使用动态光谱法检测血红蛋白的敏感波长带。基于投影重要性分析的波长筛选迈出了动态光谱走向实用的重要一步,为实现低成本在线分析打下了基础,同时也为其他领域的光谱分析提供了重要的参考和新的思路。Selection of independent variable is a hotspot in the field of quantitative spectral analysis.Efficient and easy-to-use method for wavelength selection can not only reduce the computation and improve the accuracy of analysis,but also can reduce dependency on the spectral resolution of instruments and cut cost.Wavelength selection is also an important part of the research about noninvasive measurement of blood components by spectrum technology.Dynamic Spectrum theory provides excellent ideas for researchers,but only broadband light source and high-resolution spectrograph were used in correlational studies for a long time.The large number of wavelengths needed for analysis limits the further development of Dynamic Spectrum method.In order to remove redundancy information and make the devices low-cost and integrated,the method of Wavelength selection on the basis of Variable Importance in Projection（VIP）analysis was proposed.Variables with less importance were removed and wavelengths with great explanation power were retained after VIP analysis the number of wavelengths was reduced from 586 to 64.PLS model with 64 wavelengths get a satisfactory predict result that the MREP is 1.82%.The significance test through Bootstrap method validate the selected wavelengths＇ explanation power.Moreover,the study pointed out the sensitive wavelengths in Dynamic Spectrum method for the first time.Study also took the first step toward the practical application of Dynamic Spectrum and laid the foundation of low-cost on-line analysis,and it also provided valuable references and new ideas for spectral analysis in other areas.

关 键 词：波长筛选 动态光谱 变量投影重要性 无创血液成分检测 光电容积脉搏波 

分 类 号：O433.4[机械工程—光学工程]