银杏叶提取物对大鼠背部皮肤皲裂模型的疗效观察  

Curative Effect Observation of Ginkgo Biloba Extract on Back Chapped Skin Model in Rats

作　　者：邵鸽 陈鹰[1] 潘明杰[1,2] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070 [2]湖北中医药大学药学院

出　　处：《中国药师》2016年第4期656-659,共4页China Pharmacist

基　　金：湖北省卫生厅项目(编号:JX6B27)

摘　　要：目的:探究银杏叶提取物对大鼠背部皮肤皲裂模型的疗效。方法:取SD大鼠60只随机分为6组,每组10只,制备大鼠背部皲裂模型,分别背部皮肤给药,观察高、中、低剂量银杏叶提取物组(5%、1%、0.2%),空白对照组,模型组,市售尿素组对其的疗效。HE染色观察其病理组织,采用碱水解法检测皮肤羟脯氨酸(hydroxyproline,Hyp)含量。结果:观察大鼠皮损愈合情况与病理切片综合评分情况,以及测定大鼠受损处皮肤Hyp含量,结果表明,空白对照组,模型组,尿素组,低、中、高剂量银杏叶组的大鼠综合评分分别为0,13.2,7.4,11.0,5.8,6.6,大鼠皮肤Hyp的含量分别为(4.57±0.07),(2.41±0.27),(3.29±0.57),(2.55±0.31),(3.24±0.26)和(3.37±0.24)μg·mg^(-1)。高、中剂量银杏叶提取物组与模型组差异有统计学意义(P<0.05),与市售尿素组差异无统计学意义(P>0.05)。结论:银杏叶提取物对于大鼠皮肤皲裂模型有一定的疗效,为银杏叶提取物外用制剂的研究提供了实验依据。Objective： To determine the curative effect of Ginkgo Biloba extract on the back chapped skin in SD rats. Methods：Totally 60 SD rats were randomly divided into 6 groups with 10 rats in each. One group was the blank control group,while the other five groups with the same back chapped skin were respectively treated with different therapy regimen,namely one group was the model group without any treatment,one group was urea group treated with the commercially available urea,and the last three groups were respectively treated with Ginkgo Biloba extract at the dose of 5%,1% and 0.2%. The pathological tissue was observed after HE staining,and the content of hydroxyproline（ Hyp） in skin was determined by alkaline hydrolysis. Results： The skin lesion and the score of pathological section were studied and the content of Hyp in the damaged skin was determined. The comprehensive score for the groups was0,13.2,7.4,11.0,5.8 and 6.6,respectively,and the content of Hyp was（ 4.57 ± 0.07）,（ 2.41 ± 0.27）,（ 3.29 ± 0.57）,（ 2.55 ±0.31）,（ 3.24±0.26） and（ 3.37 ± 0.24） μg ·mg^（-1）,respectively. There was significant difference in the curative effect between the Ginkgo Biloba extract groups at high dose（ 5%） and medium dose（ 1%） and the model group（ P〈0.05）,which showed no obvious difference when compared with the urea group（ P〉0.05）. Conclusion： The extract of Ginkgo Biloba has a certain effect on the back chapped skin in SD rats,which may contribute to the external preparations of Ginkgo Biloba extract.

关 键 词：银杏叶提取物 皮肤皲裂 疗效 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]