HPLC测定平肝口服液中大黄素和单参素的含量  

Determination of Emodin and Danshensu in Pinggan Oral Liquid by HPLC

作　　者：吴洋[1] 孙旭[1] 高媛[1] 李兴东[1] 马晓昱[1] 姚惠凤 陈瑶[1] 

机构地区：[1]济南军区联勤部药品仪器检验所,济南250022 [2]济南军区第150医院

出　　处：《中国药师》2016年第4期758-761,共4页China Pharmacist

基　　金：总后勤部卫生部2014年度军队医疗机构制剂标准提高科研专项课题计划(编号:14ZJZ01-1)

摘　　要：目的:建立平肝口服液中大黄素和单参素的含量测定方法。方法:采用HPLC法进行含量测定。大黄素的色谱条件为色谱柱:Agilent Eclipse XDB-C_(18)柱(150 mm×4.6 mm,5μm);流动相:甲醇-水(80∶20);检测波长:287 nm;流速:1.0 ml·min^(-1);柱温:25℃;进样量:20μl。丹参素的色谱条件为色谱柱:Agilent Eclipse SB-C_(18)柱(150 mm×4.6 mm,5μm);流动相:甲醇-1%醋酸溶液(2∶98);检测波长:280 nm;流速1.0 ml·min^(-1);柱温:30℃;进样量:20μl。结果:大黄素在26.75~428.00μg·ml^(-1)浓度范围内线性关系良好(r=0.999 9),平均回收率为102.7%,RSD为0.76%(n=6)。丹参素钠在10.17~162.77μg·ml^(-1)浓度范围内线性关系良好(r=0.999 9),平均回收率为100.5%,RSD为1.47%(n=6)。结论:该方法结果准确,重复性好,可作为该制剂的质量控制方法。Objective： To establish the method for the determination of emodin and danshensu Pinggan oral liquid. Methods： The contents were determined by HPLC. The chromatographic conditions for emodin were as follows： an Agilent Eclipse XDB-C_（18）column（ 150 mm×4.6 mm,5 μm） was used and the mobile phase was methanol-water（ 80 ∶20）. The detection wavelength was 287 nm and the flow rate was 1.0 ml·min^（-1）. The column temperature was 25℃ and the injection volume was 20 μl. The chromatographic conditions for Danshensu were as follows： an Agilent Eclipse SB-C_（18）column（ 150 mm×4.6 mm,5 μm） was used and the mobile phase was methanol-1% acetic acid solution（ 2 ∶98）. The detection wavelength was 280 nm and the flow was 1.0 ml·min^（-1）. The column temperature was 30℃ and the injection volume was 20 μl. Results： Emodin had a good linear relationship within the range of 26.75-428.00μg·ml^（-1）（ r= 0.999 9）. The average recovery was 102.7% and RSD was 0.76%（ n= 6）. Sodium Danshensu had a good linear relationship within the range of 10.17-162.77 μg·ml^（-1）（ r= 0.999 9）. The average recovery was 100.5% and RSD was 1.47%（ n= 6）.Conclusion： The method is simple,accurate and reproducible,which can be used for the quality control of the preparation.

关 键 词：平肝口服液 大黄素 丹参素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]