HPLC测定银杏总黄酮自乳化微乳中银杏总黄酮的含量  

Determination of Total Ginkgo Flavonoid in Self-emulsifying Drug Delivery System by HPLC

作　　者：廖祥茹[1] 方松[1] 史琼枝[1] 陈鹰[1] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070

出　　处：《中国药师》2016年第4期764-766,共3页China Pharmacist

基　　金：武汉总医院科研课题(编号:YZ201438)

摘　　要：目的:建立高效液相色谱法测定银杏总黄酮自乳化微乳中银杏总黄酮的含量。方法:采用菲罗门-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.4%磷酸溶液(50∶50,v/v)为流动相,流速为1.0 ml·min^(-1),检测波长为360 nm,柱温为30℃。结果:银杏总黄酮中槲皮素、山柰素和异鼠李素三种成分分离度较好,槲皮素在2.0~40.0μg·ml^(-1)质量浓度范围内,山柰素在3.0~60.0μg·ml^(-1)质量浓度范围内,异鼠李素在2.0~40.0μg·ml^(-1)质量浓度范围与峰面积呈良好的线性关系。槲皮素、山柰素和异鼠李素的平均回收率分别为98.4%,99.7%和100.5%,RSD分别为0.92%,0.62%和1.24%(n=9)。结论:该方法对银杏总黄酮自乳化微乳中三种成分分离效果较好,辅料无干扰,专属性强、稳定可靠,适用于银杏总黄酮自乳化微乳中银杏总黄酮的含量测定。Objective： To develop an HPLC method for determining the total ginkgo flavonoid in self-emulsifying drug delivery system. Methods： Effective chromatographic separation was achieved using a phenomenex C_（18）column（ 250 mm×4.6 mm,5 μm） with a mobile phase composed of methanol and water（ 0.4% phosphoric acid） with the ratio of 50 ∶50（ v / v）. The mobile phase was pumped using an isocratic HPLC system at a flow rate of 1.0 ml·min^（-1）,the detection wavelength was 360 nm and the column temperature was30 ℃. Results： The three components in the total ginkgo flavonoid were well separated by the proposed method. The linear relationship between the peak area and the concentration was promising within the range of 2.0-40.0 μg·ml^（-1）for quercetin,3.0-60.0 μg·ml^（-1）for kaempferide and 2.0-40.0 μg ·ml^（-1）for isorhamnetin. The mean recovery of quercetin,kaempferide and isorhamnetin was 98.4%,99.7% and 100.5% with RSD of 0.92%,0.62% and 1.24%（ n = 9）,respectively. Conclusion： The method is specific and stable in the determination of total ginkgo flavonoid in self-emulsifying drug delivery system.

关 键 词：银杏总黄酮 自乳化微乳 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]