添加生物炭对烤烟碳氮代谢的影响  

Effects of Biochar on Carbon and Nitrogen Metabolism of Flue-cured Tobacco

作　　者：张嘉炜[1] 杨永霞[1] 冯小虎 贾宏昉[1] 张松涛[1] 张洪映[1] 崔红[1] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]江西省烟草公司抚州市公司,江西抚州344000

出　　处：《江西农业学报》2016年第3期1-6,共6页Acta Agriculturae Jiangxi

基　　金：中国烟草总公司特色优质烟叶开发重大专项浓香型项目(110201101001 TS-01);江西烟叶物质代谢规律及调控研究(201201003)

摘　　要：为探究添加生物炭对烤烟碳氮代谢的影响,以烟草品种K326为材料,从细胞组织结构、基因表达以及物质代谢3个层面进行了系统研究。超微结构分析表明:中部叶成熟早期,不施生物炭处理的烟叶淀粉粒积累较多,随着叶片成熟降解更快。基因表达分析显示:打顶后,糖、淀粉代谢相关基因表达逐渐增强,氮代谢相关基因的表达减弱。中部叶成熟后期,添加生物炭越多,相关基因表达越强。物质代谢分析表明:打顶后,添加生物炭处理的总糖、还原糖含量降低,调制后,糖含量较高。打顶及调制后,添加600 kg/hm2生物炭处理的淀粉含量较高。添加生物炭后,总氮、烟碱含量增加,调制后差异变小,这说明添加生物炭对烟叶碳氮代谢有较大影响,进而影响烟叶品质。In order to identify the effects of biochar on carbon and nitrogen metabolism of flue-cured tobacco,taking the tobacco variety（ Nicotianatabacum L,K326） as material,conducted the systematic research from the cell tissue structure,gene expression and metabolism in three levels. Results showed that the starch were more at the earlier stages,and degraded more completely in the treatment of no applying biochar with leaf maturity. The analysis of gene expression displayed that the expression of the genes related to carbon metabolism increased gradually after topping,while the expression of the genes related to nitrogen metabolism reduced. The expression of the genes were higher by applying more biochar at the later stages. The analysis of substance contents indicated that the contents of total sugar and reducing sugar reduced after applying biochar,the contents were higher after cured. The starch contents were higher after applying 600 kg / hm2. The contents of total nitrogen and nicotine increased after applying biochar,the difference was diminished after cured,these results showed that the metabolism of tobacco carbon and nitrogen were affected by applying biochar,thus affected the quality of flue-cured tobacco.

关 键 词：烤烟 碳氮代谢 生物炭 基因表达 

分 类 号：S572[农业科学—烟草工业]