葫芦科蔬菜单倍体育种技术研究进展——花药和游离小孢子培养技术  

Advances of haploid breeding techniques of cucurbit vegetables:In vitro anther and isolated microspore culture

作　　者：董彦琪[1] 黄金华[1] 刘喜存[1] 王文英[1] 王玲燕[1] 朱红彩[1] 

机构地区：[1]河南省新乡市农业科学院,河南新乡453000

出　　处：《中国瓜菜》2016年第4期1-4,共4页China Cucurbits And Vegetables

基　　金：河南省科技开放合作项目(142106000022)

摘　　要：花药培养和游离小孢子培养技术是葫芦科作物获得单倍体胚和单倍体植株的一种重要方式。该技术在葫芦科作物育种上应用广泛,笔者综述了影响该技术的一些重要因素,如基因型、小孢子发育时期、生长环境、接种密度、培养基、生长调节剂、碳源、活性炭、三十烷醇、低温预处理、热激处理、倍性鉴定及植株再生等,为该技术的开展和应用提供可行的理论依据。The technique of in vitro anther culture and isolated microspore culture is an important tool to obtain haploid embryos and plants in cucurbit crop breeding. The technology of in vitro anther culture and isolated microspore culture is applied widely,and we reviews many factors of this technology,such as genotype,the development stage of anther,the environment of plant growth,inoculation density,medium,growth regulator,carbon source,activated carbon,triacontanol,low temperature pretreatment and heat shock treatment,ploidy identification and plant regeneration.

关 键 词：葫芦科蔬菜 单倍体育种 离体雄核发育途径 小孢子 甘露醇 三十烷醇 

分 类 号：S642[农业科学—蔬菜学]