侧脑室注射Orexin-A对大鼠胃运动的影响  

The Effect of Intracerebroventricular Injection of Orexin-A on Gastric Motility in Rats

作　　者：于武胜 郝贺玲 逄明杰[3] 郭菲菲[1] 孙向荣[1] 公衍玲[4] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理教研室,山东青岛266021 [2]青岛市第三人民医院,山东青岛266000 [3]青岛市立医院,山东青岛266000 [4]青岛科技大学,山东青岛266042

出　　处：《现代生物医学进展》2016年第7期1233-1237,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81470815;31071014;81100260;81270460;81300281);山东省优秀中青年科学家科研奖励基金项目(BS2014YY009);青岛市科技局项目(13-1-4-170-jch;14-2-3-3-nsh)

摘　　要：目的:观察侧脑室注射食欲素A(OXA)对大鼠胃运动的作用及作用途径。方法:选取成年雄性大鼠50只,随机分为5组,即生理盐水(NS)组、1μg组、5μg组、10μg组和20μg组。在胃窦和十二指肠植入应力传感器,记录大鼠胃肠自发性运动。通过侧脑室注射OXA,观察胃肠环形肌运动波形的变化和持续时间。选取10只大鼠随机分为2组,一组皮下注射NS+10μg OXA,另一组皮下注射阿托品+10μg OXA,观察OXA对胃肠运动的影响。同理选取10只大鼠分为假手术组+10μg OXA组,迷走神经切断组+10μg OXA组,观察OXA调控大鼠胃肠运动的作用途径。再选取10只大鼠,先注射NS再注射OXA拮抗剂SB334867,观察内源性OXA对胃肠运动的影响。结果:侧脑室注射OXA(1-20μg),大鼠消化间期胃和十二指肠III期收缩波消失,继而出现胃和十二指肠餐后不规则的收缩波。OXA对餐后胃肠运动的影响可被阿托品或切断迷走神经干所阻断(P<0.05)。中枢注射选择性OXA受体拮抗剂SB-334867(16μg),可增强胃消化间期III相收缩(P<0.05)。结论:中枢注射OXA可能经迷走神经胆碱能通路调控大鼠消化间期的胃肠运动,内源性OXA可能对大鼠消化间期胃肠运动具有抑制作用。Objective： To observe the effect and mechanism of intracerebroventricular（icv） injection of Orexin-A on gastric motility in rats. Methods： 50 adult male rats were randomly divided into five groups, namely normal saline（NS）, 1 μg, 5 μg, 10 μg and 20 μg group. Two strain gauge transducers were attached on the antrum and duodenum to record circular muscle contractions. Spontaneous gastroduodenal contractions were recorded in freely moving conscious rats. Through icv injection of OXA, observed the changes in the gastrointestinal circular muscle motion wave form and duration in rats. 10 rats were randomly divided into two groups, one group with subcutaneous injection of NS ＋ icv injection of 10 μg OXA, another group with subcutaneous injection of atropine ＋ icv injection of 10 μg OXA, to observe the effects on gastrointestinal motility. Similarly selecting 10 rats were divided into sham group ＋ 10 μg OXA group,vagotomy ＋ 10 μg OXA group, which was used to elucidate the neural pathways of OXA. Then selecting 10 rats, first icv injection of NS then icv injection of OXA antagonist SB334867, observed the effect of endogenous OXA on gastrointestinal motility. Results： OXA（1-20 μg） disrupted the interdigestive phase III-like contractions and induced an irregular postprandial-like motility pattern in the stomach and duodenum. The stimulatory effect of OXA on gastroduodenal postprandial-like motility pattern was abolished by atropine and truncal vagotomy（P0.05）. Central administration（icv） of selective OXA receptor antagonist, SB-334867（16 μg）, enhanced gastric spontaneous phase III-like contractions（P0.05）. Conclusion： Central administration of OXA changes GI motor pattern from interdigestive to postprandial via the vagal cholinergic pathways. Endogenous OXA may inhibit in interdigestive GI motility in rats.

关 键 词：移行性复合运动(MMC) 迷走神经切断术 OREXIN-A 

分 类 号：R338.2[医药卫生—人体生理学] R33[医药卫生—基础医学]