CREG促进小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达  

CREG Promotes Lysosome Biogenesis and Expression of Cathepsins in Mouse Peritoneal Cavity Macrophages

作　　者：孙鸣宇[1] 闫承慧[1] 田孝祥[1] 李洋[1] 韩雅玲[1] 

机构地区：[1]沈阳军区总医院全军心血管病研究所心内科,辽宁沈阳110016

出　　处：《现代生物医学进展》2016年第8期1424-1427,1471,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81130072;81370243)

摘　　要：目的:研究E1A激活基因阻遏子(Cellular repressor of E1A-stimulated genes,CREG)对小鼠腹腔巨噬细胞溶酶体发生及溶酶体组织蛋白酶表达的影响。方法:应用loss-of-function和gain-of-function模型,Lysotracker Red染色和透射电镜观察CREG对小鼠腹腔巨噬细胞溶酶体发生的影响,并通过细胞免疫荧光染色和Western blot方法,观察CREG对小鼠腹腔巨噬细胞溶酶体组织蛋白酶表达的影响。结果:Lysotracker Red染色和透射电镜证实CREG可促进小鼠腹腔巨噬细胞溶酶体发生;细胞免疫荧光染色和Western blot方法证实CREG可促进小鼠腹腔巨噬细胞溶酶体组织蛋白酶cathepsin B及cathepsin S表达。结论:CREG可促进小鼠腹腔巨噬细胞溶酶体发生及组织蛋白酶cathepsin B及cathepsin S表达。To study the effects of cellular repressor of E1 A stimulated genes（CREG） on lysosome biogenesis and expression of cathepsins in mouse peritoneal cavity macrophages. Methods： Lysotracker Red staining and transmission electron microscope were applied to observe the effect of CREG on lysosome biogenesis in mouse peritoneal cavity macrophages using gain-of-function and loss-of-function approaches. The effect of CREG on expression of cathepsins in mouse peritoneal cavity macrophages were studied by immunofluorescence staining and Western blot. Results： It was confirmed that CREG can promote lysosome biogenesis in mouse peritoneal cavity macrophages using Lysotracker Red staining and transmission electron microscope. The findings of immunofluorescence staining and Western blot provided evidence that CREG promoted expression of cathepsin B and cathepsin S in mouse peritoneal cavity macrophages. Conclusion： CREG promotes lysosome biogenesis and expression of cathepsin B and cathepsin S in mouse peritoneal cavity macrophages.

关 键 词：E1A激活基因阻遏子 巨噬细胞 溶酶体 

分 类 号：Q291[生物学—细胞生物学] R33[医药卫生—人体生理学]